TY - JOUR
T1 - Change of inverted thyroid follicle into a spheroid after embedding in a collagen gel
AU - Garbi, Corrado
AU - Tacchetti, Carlo
AU - Wollman, Seymour H.
PY - 1986
Y1 - 1986
N2 - When inverted thyroid follicles in suspension culture are embedded in a collagen gel, there is extensive reorganization of the follicle. To identify intermediate stages in the reorganization, a suspension of inverted follicles was mixed with a cold solution of collagen (0.1 mg/ml) in culture medium and the resultant was warmed and allowed to gel. Prior to embedding, the epithelial cells bounding the lumens formed a monolayer of attenuated cells with their microvilli-bearing surface in contact with the medium. The first change noted was a shrinkage of the lumen in many follicles by 18 h. The cells became cuboidal to columnar. Some of the cells had long sheet-like processes extending into the lumen in contact with those of other cells. In late stages of the reorganization, 48 h, the cells were arranged in a compact spheroid. The spheroids contained two different kinds of colloid-filled lumens, possibly of different origins, one a spherical microlumen, the other very long and narrow in section. The peripheral cells of the spheroid had a smooth plasma membrane (without microvilli) in contact with collagen. Although most of the cells in a section had a microvilli-bearing surface forming part of the boundary of a lumen, it is not certain that all cells were in contact with a lumen.
AB - When inverted thyroid follicles in suspension culture are embedded in a collagen gel, there is extensive reorganization of the follicle. To identify intermediate stages in the reorganization, a suspension of inverted follicles was mixed with a cold solution of collagen (0.1 mg/ml) in culture medium and the resultant was warmed and allowed to gel. Prior to embedding, the epithelial cells bounding the lumens formed a monolayer of attenuated cells with their microvilli-bearing surface in contact with the medium. The first change noted was a shrinkage of the lumen in many follicles by 18 h. The cells became cuboidal to columnar. Some of the cells had long sheet-like processes extending into the lumen in contact with those of other cells. In late stages of the reorganization, 48 h, the cells were arranged in a compact spheroid. The spheroids contained two different kinds of colloid-filled lumens, possibly of different origins, one a spherical microlumen, the other very long and narrow in section. The peripheral cells of the spheroid had a smooth plasma membrane (without microvilli) in contact with collagen. Although most of the cells in a section had a microvilli-bearing surface forming part of the boundary of a lumen, it is not certain that all cells were in contact with a lumen.
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U2 - 10.1016/0014-4827(86)90558-6
DO - 10.1016/0014-4827(86)90558-6
M3 - Article
C2 - 3943564
AN - SCOPUS:0022535070
VL - 163
SP - 63
EP - 77
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -