Changes in AMPA receptor-spliced variant expression and shift in AMPA receptor spontaneous desensitization pharmacology during cerebellar granule cell maturation in vitro

Patrizia Longone, Francesco Impagnatiello, Jean Marc Mienville, Erminio Costa, Alessandro Guidotti

Research output: Contribution to journalArticle

Abstract

Using appropriate internal standards, quantitative reverse transcripase- polymerase chain reaction (RT-PCR), and cerebellar granule cell (CG) in primary cultures we have quantified the expression of mRNAs encoding for GluR(1-4) DL-α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor subunits during neuronal maturation in vitro. GluR1 is the mRNA that increases during CG maturation; the expression changes of the other GluR mRNAs are minimal and the translation products of these mRNAs change with a similar pattern. During CG maturation, there is an 8- to 10-fold increase in the GluR1 FLOP mRNA and a twofold increase in the expression of FLOP mRNA for GluR4 and GluR(4C). The GluR1 FLIP mRNA increases, but by a smaller extent. We found that the GluR2 mRNA is completely edited at its Q/R site during CG maturation. The increase on the expression of GluR1 FLIP and FLOP and of GluR4 FLOP mRNA variants during development is associated with a 10- fold increase in AMPA-mediated Na+ currents and in the increased amplification of this current by 7-chloro-3-methyl-3,4 dihydro-2H-1,2,4 benzothiadiazine S-S-dioxide (IDRA21) or by 6-chloro-3,4 dihydro-3-(2- norbornen-5-yl)-7-sulfamoyl-1,2,4-benzothiadiazine 1,1 dioxide (cyclothiazide [CT]).

Original languageEnglish
Pages (from-to)23-41
Number of pages19
JournalJournal of Molecular Neuroscience
Volume11
Issue number1
Publication statusPublished - 1998

Keywords

  • AMPA receptors
  • Cerebellar granule cells
  • Cyclothiazide
  • Glutamate receptors
  • IDRA21

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry
  • Genetics

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