Changes in cellular gene expression in rat thyroid epithelial cells transformed by the Kirsten murine sarcoma virus

Vittorio de Franciscis, Vittorio E. Avvedimento, Giulia Colletta, Vincenzo Zimarino, Valeria M. Ursini, Fortunato Ciliberto, Giancarlo Vecchio

Research output: Contribution to journalArticle

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Abstract

We have exploited a recently characterized system of rat thyroid epithelial cells transformed by the wild-type (wt) and a temperature-sensitive (ts) mutant strain of the Kirsten murine sarcoma virus (Ki-MSV) in order to study the effects of the K-ras oncogene on the gene expression of differentiated thyroid epithelial cells. By using cDNAs isolated from normal thyroid glands as probes, we were able to identify three sets of cellular sequences whose expression is influenced by the v-K-ras oncogene. The first set of genes is irreversibly repressed by transformation with both the wt and the ts viruses. The second set of genes is repressed in the ts-Ki-MSV-transformed cells but not in the same cells grown at the nonpermissive temperature. A third set of genes is present at higher levels at the nonpermissive temperature than at the permissive temperature. This system has allowed us to isolate and characterize a number of cDNA clones belonging to each of these three sets of genes. These specific cDNAs are suitable probes to study phenotypical changes during transformation of epithelial cells.

Original languageEnglish
Pages (from-to)483-491
Number of pages9
JournalExperimental Cell Research
Volume171
Issue number2
DOIs
Publication statusPublished - 1987

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Kirsten murine sarcoma virus
Gene Expression
Temperature
ras Genes
Complementary DNA
Genes
Thyroid Epithelial Cells
Thyroid Gland
Clone Cells
Epithelial Cells
Viruses

ASJC Scopus subject areas

  • Cell Biology

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Changes in cellular gene expression in rat thyroid epithelial cells transformed by the Kirsten murine sarcoma virus. / de Franciscis, Vittorio; Avvedimento, Vittorio E.; Colletta, Giulia; Zimarino, Vincenzo; Ursini, Valeria M.; Ciliberto, Fortunato; Vecchio, Giancarlo.

In: Experimental Cell Research, Vol. 171, No. 2, 1987, p. 483-491.

Research output: Contribution to journalArticle

de Franciscis, Vittorio ; Avvedimento, Vittorio E. ; Colletta, Giulia ; Zimarino, Vincenzo ; Ursini, Valeria M. ; Ciliberto, Fortunato ; Vecchio, Giancarlo. / Changes in cellular gene expression in rat thyroid epithelial cells transformed by the Kirsten murine sarcoma virus. In: Experimental Cell Research. 1987 ; Vol. 171, No. 2. pp. 483-491.
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