Changes in coagulation-fibrinolysis balance in blood mononuclear cells and in gastric mucosa from patients with Helicobacter pylori infection

M. Colucci, M. R. Rossiello, A. Pentimone, P. Berloco, F. Russo, A. Di Leo, N. Semeraro

Research output: Contribution to journalArticle

Abstract

Introduction: Helicobacter pylori and some of its virulence factors stimulate human blood mononuclear cells (MNC) in vitro to produce tissue factor (TF) and plasminogen activator inhibitor-2 (PAI-2). In this study we investigated the procoagulant-fibrinolytic potential of blood MNC in patients with H. pylori infection. In the same patients we also evaluated the coagulation-fibrinolysis profile in gastric tissue and in plasma. Methods and results: The production of TF and PAI-2 was evaluated in 61 patients with dyspepsia, 31 positive and 30 negative for H. pylori infection. TF expressed by MNC and PAI-2 accumulation in cell culture medium after incubation for 20 h at 37°C were significantly higher in H. pylori(+) than in H. pylori(-) patients and were significantly correlated. TF and PAI-2 content in extracts of gastric mucosa was similar in the two groups whereas lower levels of tissue plasminogen activator (t-PA) and thrombomodulin (TM) antigens were found in the antrum of H. pylori(+) patients. No difference between the groups was observed in plasma thrombus precursor protein, prothrombin fragment 1 + 2, D-dimer, t-PA, PAI-1, TM and thrombin activatable fibrinolysis inhibitor. Conclusions: H. pylori infection is associated with functional abnormalities of blood MNC resulting in the coordinate expression of TF and antifibrinolytic activity. Changes in cell coagulation-fibrinolysis balance may represent a link between H. pylori infection and ischemic heart disease.

Original languageEnglish
Pages (from-to)471-477
Number of pages7
JournalThrombosis Research
Volume116
Issue number6
DOIs
Publication statusPublished - 2005

Fingerprint

Helicobacter Infections
Fibrinolysis
Gastric Mucosa
Helicobacter pylori
Blood Cells
Plasminogen Activator Inhibitor 2
Thromboplastin
Tissue Plasminogen Activator
Thrombomodulin
Carboxypeptidase B2
Antifibrinolytic Agents
Protein Precursors
Dyspepsia
Plasminogen Activator Inhibitor 1
Virulence Factors
Myocardial Ischemia
Culture Media
Stomach
Thrombosis
Cell Culture Techniques

Keywords

  • H. pylori infection
  • Monocytes
  • Plasminogen activator inhibitor-2
  • Thrombomodulin
  • Tissue factor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Changes in coagulation-fibrinolysis balance in blood mononuclear cells and in gastric mucosa from patients with Helicobacter pylori infection. / Colucci, M.; Rossiello, M. R.; Pentimone, A.; Berloco, P.; Russo, F.; Di Leo, A.; Semeraro, N.

In: Thrombosis Research, Vol. 116, No. 6, 2005, p. 471-477.

Research output: Contribution to journalArticle

Colucci, M. ; Rossiello, M. R. ; Pentimone, A. ; Berloco, P. ; Russo, F. ; Di Leo, A. ; Semeraro, N. / Changes in coagulation-fibrinolysis balance in blood mononuclear cells and in gastric mucosa from patients with Helicobacter pylori infection. In: Thrombosis Research. 2005 ; Vol. 116, No. 6. pp. 471-477.
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AU - Berloco, P.

AU - Russo, F.

AU - Di Leo, A.

AU - Semeraro, N.

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