Changes in cytochrome P-450 levels in hepatic microsomes after burn injuries: experimental study

A. Seccia, C. Riberti, M. De Gennaro, G. Flamini

Research output: Contribution to journalArticle

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Abstract

The microsomal fraction was extracted from the livers of a group of burned rats and a control group of unburned rats. Morphologically, this fraction corresponds to the endoplasmic reticulum membranes and is the site of the microsomal oxidative system which catalyzes numerous metabolic reactions fundamental to the detoxifying activity of the liver. Cytochrome P-450 is the terminal oxidase of the microsomal respiratory chain and it is also the haemo-protein present in the largest quantity in the microsomal fraction. Its activity is usually reflected by alterations in the biological equilibrium of its two forms (reduced and oxidized). In fact, after a burn injury, especially after 6 h, there is a modification in the cytochrome auto-oxidability as is shown by the marked increase in the NADPH-reducible fraction. The accompanying increase of the total cytochrome P-450 concentration may be due to a formation de novo and/or to a redistribution of the two different pools of cytochrome normally present. In this study, the changes in the ultrastructure of liver cells and the time course of changes in the levels of microsomal P-450 are described in detail. P-450 levels changed immediately post burn but returned to normal levels 24 h later. They could confirm the hypothesis that in a burn injury there is a 'factor' acting by a toxic mechanism; such a factor, newly formed or present as a precursor and activated by the burn, could damage the liver, or better, activate its detoxifying mechanism even in the early hours post burn.

Original languageEnglish
Pages (from-to)174-180
Number of pages7
JournalBurns
Volume6
Issue number3
DOIs
Publication statusPublished - 1980

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Microsomes
Cytochrome P-450 Enzyme System
Liver
Wounds and Injuries
Cytochromes
Poisons
Electron Transport
NADP
Endoplasmic Reticulum
Oxidoreductases
Control Groups
Membranes
Proteins

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Changes in cytochrome P-450 levels in hepatic microsomes after burn injuries : experimental study. / Seccia, A.; Riberti, C.; De Gennaro, M.; Flamini, G.

In: Burns, Vol. 6, No. 3, 1980, p. 174-180.

Research output: Contribution to journalArticle

Seccia, A. ; Riberti, C. ; De Gennaro, M. ; Flamini, G. / Changes in cytochrome P-450 levels in hepatic microsomes after burn injuries : experimental study. In: Burns. 1980 ; Vol. 6, No. 3. pp. 174-180.
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