Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia

Carlo Dufour, Elisa Ferretti, Francesca Bagnasco, Oriana Burlando, Marina Lanciotti, Ugo Ramenghi, Paola Saracco, Maria Teresa Van Lint, Daniela Longoni, Giovanni Fernando Torelli, Marta Pillon, Anna Locasciulli, Aldo Misuraca, Milena La Spina, Andrea Bacigalupo, Vito Pistoia, Anna Corcione, Johanna Svahn

Research output: Contribution to journalArticle

Abstract

Cytokine expression assessed by flow cytometry in 53 acquired aplastic anemia patients before and after combined immunosuppression (EBMT WPSAA protocols) showed that CD3 + marrow cells containing TNF-α, IFN-γ and IL4 were similar in subjects with disease at onset (DO) and responsive to treatment who had more CD3 +/TNF-α + and CD 3 +/IFN-γ + cells than normal controls. In vitro block of TNF-α and/or IFN-γ significantly increased BFU-e over baseline in 28 patients. In responsive to treatment patients only TNF-α block significantly incremented colonies over normal controls. Absolute marrow CD3 +/TNF-α + and CD3 +/IFN- γ + cells prospectively tested in a group of 21 subjects declined significantly more in Responders than in Non Responders to immunosuppression at Response Evaluation Time respect to Diagnosis. Both in Responders and in Non Responders these cells remained higher than in normal controls. This study suggests that immunosuppression does not fully clear excess TNF-α and IFN-γ from marrow of patients with good outcome and raises the hypothesis that additional cytokine blockade might be useful in immunosuppression for acquired aplastic anemia.

Original languageEnglish
Pages (from-to)1743-1747
Number of pages5
JournalHaematologica
Volume94
Issue number12
DOIs
Publication statusPublished - Dec 2009

Keywords

  • Aplastic anemia
  • IFN-γ
  • IL4
  • Immunosuppression
  • TNF-α

ASJC Scopus subject areas

  • Hematology

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    Dufour, C., Ferretti, E., Bagnasco, F., Burlando, O., Lanciotti, M., Ramenghi, U., Saracco, P., Van Lint, M. T., Longoni, D., Torelli, G. F., Pillon, M., Locasciulli, A., Misuraca, A., La Spina, M., Bacigalupo, A., Pistoia, V., Corcione, A., & Svahn, J. (2009). Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia. Haematologica, 94(12), 1743-1747. https://doi.org/10.3324/haematol.2009.007815