Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia

Carlo Dufour, Elisa Ferretti, Francesca Bagnasco, Oriana Burlando, Marina Lanciotti, Ugo Ramenghi, Paola Saracco, Maria Teresa Van Lint, Daniela Longoni, Giovanni Fernando Torelli, Marta Pillon, Anna Locasciulli, Aldo Misuraca, Milena La Spina, Andrea Bacigalupo, Vito Pistoia, Anna Corcione, Johanna Svahn

Research output: Contribution to journalArticle

Abstract

Cytokine expression assessed by flow cytometry in 53 acquired aplastic anemia patients before and after combined immunosuppression (EBMT WPSAA protocols) showed that CD3 + marrow cells containing TNF-α, IFN-γ and IL4 were similar in subjects with disease at onset (DO) and responsive to treatment who had more CD3 +/TNF-α + and CD 3 +/IFN-γ + cells than normal controls. In vitro block of TNF-α and/or IFN-γ significantly increased BFU-e over baseline in 28 patients. In responsive to treatment patients only TNF-α block significantly incremented colonies over normal controls. Absolute marrow CD3 +/TNF-α + and CD3 +/IFN- γ + cells prospectively tested in a group of 21 subjects declined significantly more in Responders than in Non Responders to immunosuppression at Response Evaluation Time respect to Diagnosis. Both in Responders and in Non Responders these cells remained higher than in normal controls. This study suggests that immunosuppression does not fully clear excess TNF-α and IFN-γ from marrow of patients with good outcome and raises the hypothesis that additional cytokine blockade might be useful in immunosuppression for acquired aplastic anemia.

Original languageEnglish
Pages (from-to)1743-1747
Number of pages5
JournalHaematologica
Volume94
Issue number12
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Aplastic Anemia
Immunosuppression
Cytokines
Bone Marrow
Interleukin-4
Flow Cytometry
Therapeutics

Keywords

  • Aplastic anemia
  • IFN-γ
  • IL4
  • Immunosuppression
  • TNF-α

ASJC Scopus subject areas

  • Hematology

Cite this

Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia. / Dufour, Carlo; Ferretti, Elisa; Bagnasco, Francesca; Burlando, Oriana; Lanciotti, Marina; Ramenghi, Ugo; Saracco, Paola; Van Lint, Maria Teresa; Longoni, Daniela; Torelli, Giovanni Fernando; Pillon, Marta; Locasciulli, Anna; Misuraca, Aldo; La Spina, Milena; Bacigalupo, Andrea; Pistoia, Vito; Corcione, Anna; Svahn, Johanna.

In: Haematologica, Vol. 94, No. 12, 12.2009, p. 1743-1747.

Research output: Contribution to journalArticle

Dufour, C, Ferretti, E, Bagnasco, F, Burlando, O, Lanciotti, M, Ramenghi, U, Saracco, P, Van Lint, MT, Longoni, D, Torelli, GF, Pillon, M, Locasciulli, A, Misuraca, A, La Spina, M, Bacigalupo, A, Pistoia, V, Corcione, A & Svahn, J 2009, 'Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia', Haematologica, vol. 94, no. 12, pp. 1743-1747. https://doi.org/10.3324/haematol.2009.007815
Dufour, Carlo ; Ferretti, Elisa ; Bagnasco, Francesca ; Burlando, Oriana ; Lanciotti, Marina ; Ramenghi, Ugo ; Saracco, Paola ; Van Lint, Maria Teresa ; Longoni, Daniela ; Torelli, Giovanni Fernando ; Pillon, Marta ; Locasciulli, Anna ; Misuraca, Aldo ; La Spina, Milena ; Bacigalupo, Andrea ; Pistoia, Vito ; Corcione, Anna ; Svahn, Johanna. / Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia. In: Haematologica. 2009 ; Vol. 94, No. 12. pp. 1743-1747.
@article{da3471bb54934cb9ad7707be07e18b8d,
title = "Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia",
abstract = "Cytokine expression assessed by flow cytometry in 53 acquired aplastic anemia patients before and after combined immunosuppression (EBMT WPSAA protocols) showed that CD3 + marrow cells containing TNF-α, IFN-γ and IL4 were similar in subjects with disease at onset (DO) and responsive to treatment who had more CD3 +/TNF-α + and CD 3 +/IFN-γ + cells than normal controls. In vitro block of TNF-α and/or IFN-γ significantly increased BFU-e over baseline in 28 patients. In responsive to treatment patients only TNF-α block significantly incremented colonies over normal controls. Absolute marrow CD3 +/TNF-α + and CD3 +/IFN- γ + cells prospectively tested in a group of 21 subjects declined significantly more in Responders than in Non Responders to immunosuppression at Response Evaluation Time respect to Diagnosis. Both in Responders and in Non Responders these cells remained higher than in normal controls. This study suggests that immunosuppression does not fully clear excess TNF-α and IFN-γ from marrow of patients with good outcome and raises the hypothesis that additional cytokine blockade might be useful in immunosuppression for acquired aplastic anemia.",
keywords = "Aplastic anemia, IFN-γ, IL4, Immunosuppression, TNF-α",
author = "Carlo Dufour and Elisa Ferretti and Francesca Bagnasco and Oriana Burlando and Marina Lanciotti and Ugo Ramenghi and Paola Saracco and {Van Lint}, {Maria Teresa} and Daniela Longoni and Torelli, {Giovanni Fernando} and Marta Pillon and Anna Locasciulli and Aldo Misuraca and {La Spina}, Milena and Andrea Bacigalupo and Vito Pistoia and Anna Corcione and Johanna Svahn",
year = "2009",
month = "12",
doi = "10.3324/haematol.2009.007815",
language = "English",
volume = "94",
pages = "1743--1747",
journal = "Haematologica",
issn = "0390-6078",
publisher = "NLM (Medline)",
number = "12",

}

TY - JOUR

T1 - Changes in cytokine profile pre- and post-immunosuppression in acquired aplastic anemia

AU - Dufour, Carlo

AU - Ferretti, Elisa

AU - Bagnasco, Francesca

AU - Burlando, Oriana

AU - Lanciotti, Marina

AU - Ramenghi, Ugo

AU - Saracco, Paola

AU - Van Lint, Maria Teresa

AU - Longoni, Daniela

AU - Torelli, Giovanni Fernando

AU - Pillon, Marta

AU - Locasciulli, Anna

AU - Misuraca, Aldo

AU - La Spina, Milena

AU - Bacigalupo, Andrea

AU - Pistoia, Vito

AU - Corcione, Anna

AU - Svahn, Johanna

PY - 2009/12

Y1 - 2009/12

N2 - Cytokine expression assessed by flow cytometry in 53 acquired aplastic anemia patients before and after combined immunosuppression (EBMT WPSAA protocols) showed that CD3 + marrow cells containing TNF-α, IFN-γ and IL4 were similar in subjects with disease at onset (DO) and responsive to treatment who had more CD3 +/TNF-α + and CD 3 +/IFN-γ + cells than normal controls. In vitro block of TNF-α and/or IFN-γ significantly increased BFU-e over baseline in 28 patients. In responsive to treatment patients only TNF-α block significantly incremented colonies over normal controls. Absolute marrow CD3 +/TNF-α + and CD3 +/IFN- γ + cells prospectively tested in a group of 21 subjects declined significantly more in Responders than in Non Responders to immunosuppression at Response Evaluation Time respect to Diagnosis. Both in Responders and in Non Responders these cells remained higher than in normal controls. This study suggests that immunosuppression does not fully clear excess TNF-α and IFN-γ from marrow of patients with good outcome and raises the hypothesis that additional cytokine blockade might be useful in immunosuppression for acquired aplastic anemia.

AB - Cytokine expression assessed by flow cytometry in 53 acquired aplastic anemia patients before and after combined immunosuppression (EBMT WPSAA protocols) showed that CD3 + marrow cells containing TNF-α, IFN-γ and IL4 were similar in subjects with disease at onset (DO) and responsive to treatment who had more CD3 +/TNF-α + and CD 3 +/IFN-γ + cells than normal controls. In vitro block of TNF-α and/or IFN-γ significantly increased BFU-e over baseline in 28 patients. In responsive to treatment patients only TNF-α block significantly incremented colonies over normal controls. Absolute marrow CD3 +/TNF-α + and CD3 +/IFN- γ + cells prospectively tested in a group of 21 subjects declined significantly more in Responders than in Non Responders to immunosuppression at Response Evaluation Time respect to Diagnosis. Both in Responders and in Non Responders these cells remained higher than in normal controls. This study suggests that immunosuppression does not fully clear excess TNF-α and IFN-γ from marrow of patients with good outcome and raises the hypothesis that additional cytokine blockade might be useful in immunosuppression for acquired aplastic anemia.

KW - Aplastic anemia

KW - IFN-γ

KW - IL4

KW - Immunosuppression

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=73149090804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73149090804&partnerID=8YFLogxK

U2 - 10.3324/haematol.2009.007815

DO - 10.3324/haematol.2009.007815

M3 - Article

C2 - 19586939

AN - SCOPUS:73149090804

VL - 94

SP - 1743

EP - 1747

JO - Haematologica

JF - Haematologica

SN - 0390-6078

IS - 12

ER -