Changes in protein composition during chick embryo retina development

M. Carabillò, E. Gerbino, H. Main, G. Calvaruso, G. Tesoriere

Research output: Contribution to journalArticle

Abstract

Two dimensional gel electrophoresis was employed to analyse the protein composition of chick embryo retina during 'in ovo' development in order to individuate some components which are typical of different stages of growth. The study of protein composition of 7-day-old chick embryo retinas revealed the presence of about eighty different soluble components. In four of these proteins the staining intensity decreased during retina development. One of these components, of 35 KD with an isoelectric point of about 5.15, was partially purified by means of preparative isoelectric focusing. Other components, which were absent at day 7 appeared during the second week of development and were clearly visualised at day 16. These results were also compared with those obtained by means of 2DGE performed on liver and brain of chick embryo at day, 7 and 16 of development.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume41
Issue number2
Publication statusPublished - Feb 1997

Keywords

  • Chick embryo retina
  • Development
  • Preparative IEF
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

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