Changes in sputum composition during 15 min of sputum induction in healthy subjects and patients with asthma and chronic obstructive pulmonary disease

Olga Toungoussova, Giovanni B. Migliori, Maria P. Foschino Barbaro, Luisa M. Esposito, Silvano Dragonieri, Giovanna E. Carpagnano, Francesco G. Salerno, Margherita Neri, Antonio Spanevello

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Introduction: The use of sputum induction by inhalation of hypertonic saline to study the cellular and biochemical composition of the airways allows noninvasive sampling of the airways content and identification of markers of airways inflammation. Objective: The present study aimed to identify possible changes in the cellular composition of induced sputum between samples obtained sequentially (three periods of 5 min each) during one sputum induction. Moreover, difference between these samples and the mixed one (mixture of samples obtained after 5, 10 and 15 min of induction) was investigated. Methods: Forty-six subjects (10 healthy volunteers, 12 patients with chronic obstructive pulmonary disease (COPD) and 24 patients with asthma) (mean age 53.0±14.0 yr, forced expiratory volume in one second (FEV1) 71.8±19.0% pred) produced sputum after three consecutive 5 min periods of hypertonic (4.5%) saline inhalation. Stained cytospins from the three periods separately and from the mixed sample were produced and analyzed. Results: The mean percentage of neutrophils, eosinophils, lymphocytes and epithelial cells did not change significantly in samples obtained consecutively after 5, 10 and 15 min of the induction procedure. There was no significant difference in the cellular composition of samples obtained after 5, 10 and 15 min of induction and the cellular composition of the mixed sample (P=0.06). Conclusion: The separate analysis of induced sputum from three consecutive sampling and the mixed sample did not demonstrate significant changes in their cellular composition. Fifteen minutes induction procedure with the fixed concentration of hypertonic saline and processing of the mixed sample can be recommended for clinical settings and clinical trials.

Original languageEnglish
Pages (from-to)1543-1548
Number of pages6
JournalRespiratory Medicine
Volume101
Issue number7
DOIs
Publication statusPublished - Jul 2007

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Sputum
Chronic Obstructive Pulmonary Disease
Healthy Volunteers
Asthma
Inhalation
Forced Expiratory Volume
Eosinophils
Neutrophils
Epithelial Cells
Clinical Trials
Lymphocytes
Inflammation

Keywords

  • Asthma
  • Chronic obstructive pulmonary disease
  • Induced sputum

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Changes in sputum composition during 15 min of sputum induction in healthy subjects and patients with asthma and chronic obstructive pulmonary disease. / Toungoussova, Olga; Migliori, Giovanni B.; Foschino Barbaro, Maria P.; Esposito, Luisa M.; Dragonieri, Silvano; Carpagnano, Giovanna E.; Salerno, Francesco G.; Neri, Margherita; Spanevello, Antonio.

In: Respiratory Medicine, Vol. 101, No. 7, 07.2007, p. 1543-1548.

Research output: Contribution to journalArticle

Toungoussova, Olga ; Migliori, Giovanni B. ; Foschino Barbaro, Maria P. ; Esposito, Luisa M. ; Dragonieri, Silvano ; Carpagnano, Giovanna E. ; Salerno, Francesco G. ; Neri, Margherita ; Spanevello, Antonio. / Changes in sputum composition during 15 min of sputum induction in healthy subjects and patients with asthma and chronic obstructive pulmonary disease. In: Respiratory Medicine. 2007 ; Vol. 101, No. 7. pp. 1543-1548.
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abstract = "Introduction: The use of sputum induction by inhalation of hypertonic saline to study the cellular and biochemical composition of the airways allows noninvasive sampling of the airways content and identification of markers of airways inflammation. Objective: The present study aimed to identify possible changes in the cellular composition of induced sputum between samples obtained sequentially (three periods of 5 min each) during one sputum induction. Moreover, difference between these samples and the mixed one (mixture of samples obtained after 5, 10 and 15 min of induction) was investigated. Methods: Forty-six subjects (10 healthy volunteers, 12 patients with chronic obstructive pulmonary disease (COPD) and 24 patients with asthma) (mean age 53.0±14.0 yr, forced expiratory volume in one second (FEV1) 71.8±19.0{\%} pred) produced sputum after three consecutive 5 min periods of hypertonic (4.5{\%}) saline inhalation. Stained cytospins from the three periods separately and from the mixed sample were produced and analyzed. Results: The mean percentage of neutrophils, eosinophils, lymphocytes and epithelial cells did not change significantly in samples obtained consecutively after 5, 10 and 15 min of the induction procedure. There was no significant difference in the cellular composition of samples obtained after 5, 10 and 15 min of induction and the cellular composition of the mixed sample (P=0.06). Conclusion: The separate analysis of induced sputum from three consecutive sampling and the mixed sample did not demonstrate significant changes in their cellular composition. Fifteen minutes induction procedure with the fixed concentration of hypertonic saline and processing of the mixed sample can be recommended for clinical settings and clinical trials.",
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AU - Toungoussova, Olga

AU - Migliori, Giovanni B.

AU - Foschino Barbaro, Maria P.

AU - Esposito, Luisa M.

AU - Dragonieri, Silvano

AU - Carpagnano, Giovanna E.

AU - Salerno, Francesco G.

AU - Neri, Margherita

AU - Spanevello, Antonio

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N2 - Introduction: The use of sputum induction by inhalation of hypertonic saline to study the cellular and biochemical composition of the airways allows noninvasive sampling of the airways content and identification of markers of airways inflammation. Objective: The present study aimed to identify possible changes in the cellular composition of induced sputum between samples obtained sequentially (three periods of 5 min each) during one sputum induction. Moreover, difference between these samples and the mixed one (mixture of samples obtained after 5, 10 and 15 min of induction) was investigated. Methods: Forty-six subjects (10 healthy volunteers, 12 patients with chronic obstructive pulmonary disease (COPD) and 24 patients with asthma) (mean age 53.0±14.0 yr, forced expiratory volume in one second (FEV1) 71.8±19.0% pred) produced sputum after three consecutive 5 min periods of hypertonic (4.5%) saline inhalation. Stained cytospins from the three periods separately and from the mixed sample were produced and analyzed. Results: The mean percentage of neutrophils, eosinophils, lymphocytes and epithelial cells did not change significantly in samples obtained consecutively after 5, 10 and 15 min of the induction procedure. There was no significant difference in the cellular composition of samples obtained after 5, 10 and 15 min of induction and the cellular composition of the mixed sample (P=0.06). Conclusion: The separate analysis of induced sputum from three consecutive sampling and the mixed sample did not demonstrate significant changes in their cellular composition. Fifteen minutes induction procedure with the fixed concentration of hypertonic saline and processing of the mixed sample can be recommended for clinical settings and clinical trials.

AB - Introduction: The use of sputum induction by inhalation of hypertonic saline to study the cellular and biochemical composition of the airways allows noninvasive sampling of the airways content and identification of markers of airways inflammation. Objective: The present study aimed to identify possible changes in the cellular composition of induced sputum between samples obtained sequentially (three periods of 5 min each) during one sputum induction. Moreover, difference between these samples and the mixed one (mixture of samples obtained after 5, 10 and 15 min of induction) was investigated. Methods: Forty-six subjects (10 healthy volunteers, 12 patients with chronic obstructive pulmonary disease (COPD) and 24 patients with asthma) (mean age 53.0±14.0 yr, forced expiratory volume in one second (FEV1) 71.8±19.0% pred) produced sputum after three consecutive 5 min periods of hypertonic (4.5%) saline inhalation. Stained cytospins from the three periods separately and from the mixed sample were produced and analyzed. Results: The mean percentage of neutrophils, eosinophils, lymphocytes and epithelial cells did not change significantly in samples obtained consecutively after 5, 10 and 15 min of the induction procedure. There was no significant difference in the cellular composition of samples obtained after 5, 10 and 15 min of induction and the cellular composition of the mixed sample (P=0.06). Conclusion: The separate analysis of induced sputum from three consecutive sampling and the mixed sample did not demonstrate significant changes in their cellular composition. Fifteen minutes induction procedure with the fixed concentration of hypertonic saline and processing of the mixed sample can be recommended for clinical settings and clinical trials.

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KW - Chronic obstructive pulmonary disease

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