Changes in the hemagglutinins and neuraminidases of human influenza B viruses isolated in Italy during the 2001-02, 2002-03, and 2003-04 seasons

Simona Puzelli, Fabiola Frezza, Concetta Fabiani, Filippo Ansaldi, Laura Campitelli, Yi Pu Lin, Victoria Gregory, Michael Bennett, Pierlanfranco D'Agaro, Cesare Campello, Pietro Crovari, Alan Hay, Isabella Donatelli

Research output: Contribution to journalArticle

Abstract

Throughout most of the last decade, B/Yamagata/ 16/88-lineage influenza viruses were predominant among the B isolates circulating worldwide, whereas B/Victoria/2/87-lineage viruses were isolated infrequently and restricted geographically to eastern Asia. During the 2001-02 influenza season, B/Victoria/2/87-lineage viruses re-emerged in North America and Europe and spread worldwide. Virological surveillance in Italy during that season showed wide circulation of influenza B viruses, of which most were antigenically related to the B/Sichuan/379/99 (Yamagata-lineage) vaccine strain, together with a smaller number of B viruses antigenically similar to B/HongKong/330/01, a recent B/Victoria/2/87-lineage antigenic variant. In the subsequent 2002-03 epidemic season, B viruses with a Victoria-lineage hemagglutinin (HA), more closely related to that of B/Shandong/7/97, were isolated exclusively. Similar strains have continued to predominate among the few B viruses isolated in Italy during last season (2003-04), although most influenza B viruses, isolated sporadically elsewhere in Europe, again belong to the Yamagata-lineage. In the present study, phylogenetic analyses of the HA and neuraminidase (NA) genes of representative B strains, isolated throughout Italy during 2001-04, showed that during the first influenza season the NA genes, as well as the H A genes, separated into the two distinct clades, the Yamagata- and Victoria-lineages, and showed no evidence of genetic reassortment. On the contrary, all the B viruses isolated in the 2002-03 and most of those isolated in the 2003-04 epidemic season were "Victoria HA-Yamagata NA" reassortants similar to those isolated in other parts of the world, showing that these reassortants became established in the human population. The frequency of reassortment between HA and NA of distinct lineages and sublineages highlights again the importance of detailed molecular analyses of both surface glycoproteins in understanding the evolution of influenza B viruses.

Original languageEnglish
Pages (from-to)629-640
Number of pages12
JournalJournal of Medical Virology
Volume74
Issue number4
DOIs
Publication statusPublished - Dec 2004

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Influenza B virus
Victoria
Hemagglutinins
Neuraminidase
Human Influenza
Italy
Cercopithecine Herpesvirus 1
vif Genes
Viruses
Far East
Membrane Glycoproteins
Hong Kong
North America
Orthomyxoviridae
Genes
Vaccines
Population

Keywords

  • Evolution
  • Hemagglutinin
  • Influenza B viruses
  • Neuraminidase

ASJC Scopus subject areas

  • Virology

Cite this

Changes in the hemagglutinins and neuraminidases of human influenza B viruses isolated in Italy during the 2001-02, 2002-03, and 2003-04 seasons. / Puzelli, Simona; Frezza, Fabiola; Fabiani, Concetta; Ansaldi, Filippo; Campitelli, Laura; Lin, Yi Pu; Gregory, Victoria; Bennett, Michael; D'Agaro, Pierlanfranco; Campello, Cesare; Crovari, Pietro; Hay, Alan; Donatelli, Isabella.

In: Journal of Medical Virology, Vol. 74, No. 4, 12.2004, p. 629-640.

Research output: Contribution to journalArticle

Puzelli, S, Frezza, F, Fabiani, C, Ansaldi, F, Campitelli, L, Lin, YP, Gregory, V, Bennett, M, D'Agaro, P, Campello, C, Crovari, P, Hay, A & Donatelli, I 2004, 'Changes in the hemagglutinins and neuraminidases of human influenza B viruses isolated in Italy during the 2001-02, 2002-03, and 2003-04 seasons', Journal of Medical Virology, vol. 74, no. 4, pp. 629-640. https://doi.org/10.1002/jmv.20225
Puzelli, Simona ; Frezza, Fabiola ; Fabiani, Concetta ; Ansaldi, Filippo ; Campitelli, Laura ; Lin, Yi Pu ; Gregory, Victoria ; Bennett, Michael ; D'Agaro, Pierlanfranco ; Campello, Cesare ; Crovari, Pietro ; Hay, Alan ; Donatelli, Isabella. / Changes in the hemagglutinins and neuraminidases of human influenza B viruses isolated in Italy during the 2001-02, 2002-03, and 2003-04 seasons. In: Journal of Medical Virology. 2004 ; Vol. 74, No. 4. pp. 629-640.
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AU - Fabiani, Concetta

AU - Ansaldi, Filippo

AU - Campitelli, Laura

AU - Lin, Yi Pu

AU - Gregory, Victoria

AU - Bennett, Michael

AU - D'Agaro, Pierlanfranco

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AU - Crovari, Pietro

AU - Hay, Alan

AU - Donatelli, Isabella

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