Alterazione del bilancio Th1/Th2 nei pazienti in trattamento emodialitico: effetto di differenti membrane.

Translated title of the contribution: Changes is Th1/Th2 balance in hemodialysis patients: effect of different membranes

C. Libetta, M. Zucchi, E. Gori, V. Sepe, F. Galli, P. Pisacco, G. Bezoari, F. Milanesi, F. Meloni, A. Dal Canton

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

BACKGROUND: Hemodialysis (HD) patients present an immunodeficiency that is mainly related to the defect of cell-mediated immunity. We have previously shown the polarisation of T-helper cells toward the phenotype in HD treatment with cuprophan membrane. In the present study, we have examined the effect of a Vitamin E-coated dialyser (Excebrane, VE) on the activity of the two Th subsets. METHODS: We studied 8 healthy controls and 10 patients on RDT for at least 6 months with cellulose membrane (AC), then switched to HD-VE. Blood was collected from HD patients while on treatment with AC, and after 1 year of treatment with VE. CD4+ cells were isolated from peripheral blood mononuclear cells (PBMC) by negative selection, treating PBMC with a cocktail of anti-CD8, -CD16, -CD19, -CD36 and -CD56 antibodies labelled with magnetic beads, and passing them through a magnetic field. The collected Th cells were cultured for 48 h with and without phytohemagglutinin (PHA). IFNgamma and IL-4 were measured in the supernatant using the ELISA assay. RESULTS: The constitutive release of IL-4 by CD4+ cells was abated significantly by treatment with VE. IFNgamma released by mitogen-stimulated CD4+ recovered with VE. CONCLUSIONS: This study demonstrates that treatment with vitamin E-coated dialyser improves the defect of PBMC function associated with cellulose membrane dialyser consisting of altered spontaneous and mitogen-stimulated cytokine release. The effects of vitamin E-coated filter, in particular the recovery of reactive IFNgamma production by Th1 cells and the restriction of spontaneous IL-4 release by Th2 cells may have clinical importance.

Original languageItalian
Pages (from-to)34-39
Number of pages6
JournalGiornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia
Volume21
Issue number1
Publication statusPublished - Jan 2004

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Th1-Th2 Balance
Renal Dialysis
Vitamin E
Membranes
Interleukin-4
Blood Cells
Mitogens
Cellulose
Therapeutics
Th2 Cells
Th1 Cells
Phytohemagglutinins
Magnetic Fields
Helper-Inducer T-Lymphocytes
Cellular Immunity
Cultured Cells
Enzyme-Linked Immunosorbent Assay
Cytokines
Phenotype
Antibodies

ASJC Scopus subject areas

  • Nephrology

Cite this

Alterazione del bilancio Th1/Th2 nei pazienti in trattamento emodialitico : effetto di differenti membrane. / Libetta, C.; Zucchi, M.; Gori, E.; Sepe, V.; Galli, F.; Pisacco, P.; Bezoari, G.; Milanesi, F.; Meloni, F.; Dal Canton, A.

In: Giornale italiano di nefrologia : organo ufficiale della Società italiana di nefrologia, Vol. 21, No. 1, 01.2004, p. 34-39.

Research output: Contribution to journalArticle

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abstract = "BACKGROUND: Hemodialysis (HD) patients present an immunodeficiency that is mainly related to the defect of cell-mediated immunity. We have previously shown the polarisation of T-helper cells toward the phenotype in HD treatment with cuprophan membrane. In the present study, we have examined the effect of a Vitamin E-coated dialyser (Excebrane, VE) on the activity of the two Th subsets. METHODS: We studied 8 healthy controls and 10 patients on RDT for at least 6 months with cellulose membrane (AC), then switched to HD-VE. Blood was collected from HD patients while on treatment with AC, and after 1 year of treatment with VE. CD4+ cells were isolated from peripheral blood mononuclear cells (PBMC) by negative selection, treating PBMC with a cocktail of anti-CD8, -CD16, -CD19, -CD36 and -CD56 antibodies labelled with magnetic beads, and passing them through a magnetic field. The collected Th cells were cultured for 48 h with and without phytohemagglutinin (PHA). IFNgamma and IL-4 were measured in the supernatant using the ELISA assay. RESULTS: The constitutive release of IL-4 by CD4+ cells was abated significantly by treatment with VE. IFNgamma released by mitogen-stimulated CD4+ recovered with VE. CONCLUSIONS: This study demonstrates that treatment with vitamin E-coated dialyser improves the defect of PBMC function associated with cellulose membrane dialyser consisting of altered spontaneous and mitogen-stimulated cytokine release. The effects of vitamin E-coated filter, in particular the recovery of reactive IFNgamma production by Th1 cells and the restriction of spontaneous IL-4 release by Th2 cells may have clinical importance.",
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AU - Zucchi, M.

AU - Gori, E.

AU - Sepe, V.

AU - Galli, F.

AU - Pisacco, P.

AU - Bezoari, G.

AU - Milanesi, F.

AU - Meloni, F.

AU - Dal Canton, A.

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