Changes of biological features in breast cancer cells determined by primary chemotherapy

Antonio Frassoldati, Francesca Adami, Chiara Banzi, Mario Criscuolo, Lino Piccinini, Vittorio Silingardi

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Abstract

Background. To evaluate the changes in the biological features of breast cancer cells induced by primary chemotherapy (PCT) and their possible relationship with the response to therapy, we performed an extensive immunohistochemical study before and after PCT. Patients and methods. PCT was administered to 29 women with breast cancer. On specimens obtained by tru-cut and post-chemotherapy surgery we analyzed the following parameters: histology, histologic grade, apoptotic index, hormone receptor levels, Ki67, PCNA, EGFr, bcl-2, p53, p170. The significance of the changes induced by PCT and their correlations with the type of response were evaluated. Results. Twelve patients achieved a partial response with PCT. No baseline biological parameter correlated with the type of response. After PCT we observed a significant increase in the apoptotic index (p = 0.000), PCNA (p = 0.036), EGFr (p = 0.005), and p170 expression (p = 0.001), regardless of the type of chemotherapy administered (anthracyclines, 25 cases, or CMF, 4 cases). Responder patients displayed a significant decrease in ER levels (p = 0.015), whereas in non responders there was an increase in PCNA (p = 0.008) and EGFr expression (p =0.002). The apoptotic index and p170 expression rose after PCT regardless of the type of response. Conclusions. PCT induced significant variations in the phenotype of breast cancer cells. These changes might reflect the selection of new neoplastic clones with different biological properties and so could facilitate the choice of appropriate chemotherapy agents.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalBreast Cancer Research and Treatment
Volume44
Issue number3
DOIs
Publication statusPublished - 1997

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Breast Neoplasms
Drug Therapy
Proliferating Cell Nuclear Antigen
Anthracyclines
Histology
Clone Cells
Hormones
Phenotype

Keywords

  • Biological features
  • Breast cancer
  • Primary chemotherapy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Changes of biological features in breast cancer cells determined by primary chemotherapy. / Frassoldati, Antonio; Adami, Francesca; Banzi, Chiara; Criscuolo, Mario; Piccinini, Lino; Silingardi, Vittorio.

In: Breast Cancer Research and Treatment, Vol. 44, No. 3, 1997, p. 185-192.

Research output: Contribution to journalArticle

Frassoldati, Antonio ; Adami, Francesca ; Banzi, Chiara ; Criscuolo, Mario ; Piccinini, Lino ; Silingardi, Vittorio. / Changes of biological features in breast cancer cells determined by primary chemotherapy. In: Breast Cancer Research and Treatment. 1997 ; Vol. 44, No. 3. pp. 185-192.
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