TY - JOUR
T1 - Chaperonins dependent increase of Cu,Zn Superoxide dismutase production in Escherichia coli
AU - Battistoni, Andrea
AU - Carrí, M. Teresa
AU - Steinkühler, Christian
AU - Rotilio, Giuseppe
PY - 1993/5/3
Y1 - 1993/5/3
N2 - Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn Superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn Superoxide dismutase in vitro, confirming that GroEL can interact with β-stranded proteins.
AB - Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn Superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn Superoxide dismutase in vitro, confirming that GroEL can interact with β-stranded proteins.
KW - Chaperonin
KW - Cu,Zn Superoxide dismutase
KW - Protein folding
UR - http://www.scopus.com/inward/record.url?scp=0027503574&partnerID=8YFLogxK
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U2 - 10.1016/0014-5793(93)81099-L
DO - 10.1016/0014-5793(93)81099-L
M3 - Article
C2 - 8097729
AN - SCOPUS:0027503574
VL - 322
SP - 6
EP - 9
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -