Chaperonins dependent increase of Cu,Zn Superoxide dismutase production in Escherichia coli

Andrea Battistoni, M. Teresa Carrí, Christian Steinkühler, Giuseppe Rotilio

Research output: Contribution to journalArticlepeer-review

Abstract

Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn Superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn Superoxide dismutase in vitro, confirming that GroEL can interact with β-stranded proteins.

Original languageEnglish
Pages (from-to)6-9
Number of pages4
JournalFEBS Letters
Volume322
Issue number1
DOIs
Publication statusPublished - May 3 1993

Keywords

  • Chaperonin
  • Cu,Zn Superoxide dismutase
  • Protein folding

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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