Chapter 15 Analysis of Neuronal Cell Death in Mammals

Marcello D'Amelio, Virve Cavallucci, Adamo Diamantini, Francesco Cecconi

Research output: Contribution to journalArticle

Abstract

Apoptosis, often defined as programmed cell death, plays a very important role in many physiologic and pathologic conditions. Therefore, detecting apoptotic cells or monitoring the cells progressing to apoptosis is an essential step in basic and/or applied research. Apoptosis is characterized by many biologic and morphologic changes of cells, for example, cytochrome c release from mitochondria, activation of caspases, DNA fragmentation, membrane blebbing, and formation of apoptotic bodies. On the basis of these changes, various assays have been designed to detect or quantify apoptotic cells. The goal of this chapter is to provide readers with a scientific guide to proven methods that highlight the current strategies for detecting apoptosis in the nervous system.

Original languageEnglish
Pages (from-to)259-276
Number of pages18
JournalMethods in Enzymology
Volume446
DOIs
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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