Chapter Eighteen Methods to Dissect Mitochondrial Membrane Permeabilization in the Course of Apoptosis

Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Claire Séror, Emilie Hangen, Jean Luc Perfettini, Nazanine Modjtahedi, Guido Kroemer

Research output: Contribution to journalArticle

Abstract

In several paradigms of cell death, mitochondrial membrane permeabilization (MMP) delimits the frontier between life and death. Mitochondria control the intrinsic pathway of apoptosis and participate in the extrinsic pathway. Moreover, they have been implicated in nonapoptotic cell death modalities. Irrespective of its initiation at the inner or the outer mitochondrial membrane (IM and OM, respectively), MMP culminates in the functional (dissipation of the mitochondrial transmembrane potential, shutdown of ATP synthesis, redox imbalance) and structural (reorganization of cristae, release of toxic intermembrane space proteins into the cytosol) collapse of mitochondria. This has a profound impact on cellular metabolism, activates caspase-dependent and -independent executioner mechanisms, and finally results in the demise of the cell. However, the partial and/or temporary permeabilization of one or both mitochondrial membranes is not always a prelude to cell death. This chapter proposes a method and several guidelines to discriminate between IM and OM permeabilization and to identify MMP that does indeed precede cell death. This approach relies on the integration of currently available techniques and may be easily introduced in the laboratory routine for a more precise detection of cell death.

Original languageEnglish
Pages (from-to)355-374
Number of pages20
JournalMethods in Enzymology
Volume442
DOIs
Publication statusPublished - 2008

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Mitochondrial Membranes
Cell death
Cell Death
Apoptosis
Membranes
Mitochondria
Poisons
Caspases
Metabolism
Membrane Potentials
Cytosol
Oxidation-Reduction
Adenosine Triphosphate
Guidelines
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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Chapter Eighteen Methods to Dissect Mitochondrial Membrane Permeabilization in the Course of Apoptosis. / Galluzzi, Lorenzo; Vitale, Ilio; Kepp, Oliver; Séror, Claire; Hangen, Emilie; Perfettini, Jean Luc; Modjtahedi, Nazanine; Kroemer, Guido.

In: Methods in Enzymology, Vol. 442, 2008, p. 355-374.

Research output: Contribution to journalArticle

Galluzzi, L, Vitale, I, Kepp, O, Séror, C, Hangen, E, Perfettini, JL, Modjtahedi, N & Kroemer, G 2008, 'Chapter Eighteen Methods to Dissect Mitochondrial Membrane Permeabilization in the Course of Apoptosis', Methods in Enzymology, vol. 442, pp. 355-374. https://doi.org/10.1016/S0076-6879(08)01418-3
Galluzzi, Lorenzo ; Vitale, Ilio ; Kepp, Oliver ; Séror, Claire ; Hangen, Emilie ; Perfettini, Jean Luc ; Modjtahedi, Nazanine ; Kroemer, Guido. / Chapter Eighteen Methods to Dissect Mitochondrial Membrane Permeabilization in the Course of Apoptosis. In: Methods in Enzymology. 2008 ; Vol. 442. pp. 355-374.
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