Characterisation and chromosomal localisation of the rat α- and β-adducin-encoding genes

Grazia Tripodi, Giorgio Casari, Sergio Tisminetzky, Giuseppe Bianchi, Giulia Devescovi, Andres Muro, Renu Tuteja, Francisco E. Baralle

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Abstract

A polymorphism in the genes encoding α- and β-adducin (ADD) was described as being associated with blood-pressure variation in a genetically hypertensive strain of rats (MHS). ADD is a cytoskeletal heterodimeric protein which may be involved in cellular signal transduction and interacts with other membrane skeleton proteins which affect ion transport across the cell membrane. The cDNA encoding the α- subunit of rat ADD was isolated using PCR methods. The cDNA consists of about 3900 bp and encodes a protein of 735 amino acids (aa) which shows 91% aa identity with the human counterpart. In spleen and kidney, three alternative spliced exons were found by PCR amplification and confirmed by RNase protection analysis. 17 inbred rat strains were genotyped for the polymorphism in the α- and β-ADD genes. Chromosomal localisation mapped rat α-ADD on chromosome 14 and rat β-ADD on chromosome 4.

Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalGene
Volume166
Issue number2
DOIs
Publication statusPublished - Dec 12 1995

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Keywords

  • alternative splicing
  • chromosome 14
  • chromosome 4
  • cytoskeletal protein
  • hypertension
  • RNase protection

ASJC Scopus subject areas

  • Genetics

Cite this

Tripodi, G., Casari, G., Tisminetzky, S., Bianchi, G., Devescovi, G., Muro, A., Tuteja, R., & Baralle, F. E. (1995). Characterisation and chromosomal localisation of the rat α- and β-adducin-encoding genes. Gene, 166(2), 307-311. https://doi.org/10.1016/0378-1119(95)00590-0