Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy

Lavinia Fabeni, Claudia Alteri, Giulia Berno, Rossana Scutari, Nicoletta Orchi, Gabriella De Carli, Ada Bertoli, Luca Carioti, Caterina Gori, Federica Forbici, Romina Salpini, Alessandra Vergori, Roberta Gagliardini, Stefania Cicalini, Annalisa Mondi, Carmela Pinnetti, Laura Mazzuti, Ombretta Turriziani, Manuela Colafigli, Vanni Borghi & 10 others Francesco Montella, Alfredo Pennica, Miriam Lichtner, Enrico Girardi, Massimo Andreoni, Cristina Mussini, Andrea Antinori, Francesca Ceccherini-Silberstein, Carlo Federico Perno, Maria Mercedes Santoro

Research output: Contribution to journalArticle

Abstract

Objective: We evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 in Italy. Methods: Phylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms and identify MTCs. MTCs were divided into small (SMTCs, 2-3 sequences), medium (MMTCs, 4-9 sequences) and large (LMTCs, ≥10 sequences). Factors associated with MTCs were evaluated using logistic regression analysis. Results: 145 MTCs were identified and involved 666 individuals (35.2%); 319 of them (16.9%) were included in 13 LMTCs, 111 (5.9%) in 20 MMTCs and 236 (12.5%) in 112 SMTCs. Compared with individuals out of MTCs, individuals involved in MTCs were prevalently Italian (72.7% vs 30.9%, p<0.001), male (82.9% vs 62.3%, p<0.001) and men who have sex with men (MSM) (43.5% vs 14.5%, p<0.001). Individuals in MTCs were also younger (median (IQR) years: 41 (35-49) vs 43 (36-51), p<0.001) and had higher CD4 cell count in comparison with individuals out of MTCs (median (IQR) cells/mm 3 : 10 9 /L 0.4 (0.265-0.587) vs 0.246 (0.082-0.417), p<0.001). The viral load remained stable between the two groups (median (IQR) log 10 copies/mL: 4.8 (4.2-5.5) vs 5.0 (4.3-5.5), p=0.87). Logistic regression confirmed that certain factors such as being MSM, of Italian origin, younger age and higher CD4 cell count were significantly associated with MTCs. Conclusions: Our findings show that HIV-1 newly diagnosed individuals infected with non-B subtypes are involved in several MTCs in Italy. These MTCs include mainly Italians and MSM and highlight the complex phenomenon characterising the HIV-1 spread. This is important especially in view of monitoring the HIV epidemic and guiding the public health response.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalSexually Transmitted Infections
DOIs
Publication statusPublished - Jan 1 2019

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Italy
HIV-1
CD4 Lymphocyte Count
Logistic Models
Viral Load
Public Health
Regression Analysis
HIV

Keywords

  • HIV
  • molecular epidemiology
  • transmission dynamics
  • transmission networks
  • virology HIV

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

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Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy. / Fabeni, Lavinia; Alteri, Claudia; Berno, Giulia; Scutari, Rossana; Orchi, Nicoletta; De Carli, Gabriella; Bertoli, Ada; Carioti, Luca; Gori, Caterina; Forbici, Federica; Salpini, Romina; Vergori, Alessandra; Gagliardini, Roberta; Cicalini, Stefania; Mondi, Annalisa; Pinnetti, Carmela; Mazzuti, Laura; Turriziani, Ombretta; Colafigli, Manuela; Borghi, Vanni; Montella, Francesco; Pennica, Alfredo; Lichtner, Miriam; Girardi, Enrico; Andreoni, Massimo; Mussini, Cristina; Antinori, Andrea; Ceccherini-Silberstein, Francesca; Perno, Carlo Federico; Santoro, Maria Mercedes.

In: Sexually Transmitted Infections, 01.01.2019, p. 1-7.

Research output: Contribution to journalArticle

Fabeni, L, Alteri, C, Berno, G, Scutari, R, Orchi, N, De Carli, G, Bertoli, A, Carioti, L, Gori, C, Forbici, F, Salpini, R, Vergori, A, Gagliardini, R, Cicalini, S, Mondi, A, Pinnetti, C, Mazzuti, L, Turriziani, O, Colafigli, M, Borghi, V, Montella, F, Pennica, A, Lichtner, M, Girardi, E, Andreoni, M, Mussini, C, Antinori, A, Ceccherini-Silberstein, F, Perno, CF & Santoro, MM 2019, 'Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy', Sexually Transmitted Infections, pp. 1-7. https://doi.org/10.1136/sextrans-2019-054017
Fabeni, Lavinia ; Alteri, Claudia ; Berno, Giulia ; Scutari, Rossana ; Orchi, Nicoletta ; De Carli, Gabriella ; Bertoli, Ada ; Carioti, Luca ; Gori, Caterina ; Forbici, Federica ; Salpini, Romina ; Vergori, Alessandra ; Gagliardini, Roberta ; Cicalini, Stefania ; Mondi, Annalisa ; Pinnetti, Carmela ; Mazzuti, Laura ; Turriziani, Ombretta ; Colafigli, Manuela ; Borghi, Vanni ; Montella, Francesco ; Pennica, Alfredo ; Lichtner, Miriam ; Girardi, Enrico ; Andreoni, Massimo ; Mussini, Cristina ; Antinori, Andrea ; Ceccherini-Silberstein, Francesca ; Perno, Carlo Federico ; Santoro, Maria Mercedes. / Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy. In: Sexually Transmitted Infections. 2019 ; pp. 1-7.
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abstract = "Objective: We evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 in Italy. Methods: Phylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms and identify MTCs. MTCs were divided into small (SMTCs, 2-3 sequences), medium (MMTCs, 4-9 sequences) and large (LMTCs, ≥10 sequences). Factors associated with MTCs were evaluated using logistic regression analysis. Results: 145 MTCs were identified and involved 666 individuals (35.2{\%}); 319 of them (16.9{\%}) were included in 13 LMTCs, 111 (5.9{\%}) in 20 MMTCs and 236 (12.5{\%}) in 112 SMTCs. Compared with individuals out of MTCs, individuals involved in MTCs were prevalently Italian (72.7{\%} vs 30.9{\%}, p<0.001), male (82.9{\%} vs 62.3{\%}, p<0.001) and men who have sex with men (MSM) (43.5{\%} vs 14.5{\%}, p<0.001). Individuals in MTCs were also younger (median (IQR) years: 41 (35-49) vs 43 (36-51), p<0.001) and had higher CD4 cell count in comparison with individuals out of MTCs (median (IQR) cells/mm 3 : 10 9 /L 0.4 (0.265-0.587) vs 0.246 (0.082-0.417), p<0.001). The viral load remained stable between the two groups (median (IQR) log 10 copies/mL: 4.8 (4.2-5.5) vs 5.0 (4.3-5.5), p=0.87). Logistic regression confirmed that certain factors such as being MSM, of Italian origin, younger age and higher CD4 cell count were significantly associated with MTCs. Conclusions: Our findings show that HIV-1 newly diagnosed individuals infected with non-B subtypes are involved in several MTCs in Italy. These MTCs include mainly Italians and MSM and highlight the complex phenomenon characterising the HIV-1 spread. This is important especially in view of monitoring the HIV epidemic and guiding the public health response.",
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author = "Lavinia Fabeni and Claudia Alteri and Giulia Berno and Rossana Scutari and Nicoletta Orchi and {De Carli}, Gabriella and Ada Bertoli and Luca Carioti and Caterina Gori and Federica Forbici and Romina Salpini and Alessandra Vergori and Roberta Gagliardini and Stefania Cicalini and Annalisa Mondi and Carmela Pinnetti and Laura Mazzuti and Ombretta Turriziani and Manuela Colafigli and Vanni Borghi and Francesco Montella and Alfredo Pennica and Miriam Lichtner and Enrico Girardi and Massimo Andreoni and Cristina Mussini and Andrea Antinori and Francesca Ceccherini-Silberstein and Perno, {Carlo Federico} and Santoro, {Maria Mercedes}",
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TY - JOUR

T1 - Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy

AU - Fabeni, Lavinia

AU - Alteri, Claudia

AU - Berno, Giulia

AU - Scutari, Rossana

AU - Orchi, Nicoletta

AU - De Carli, Gabriella

AU - Bertoli, Ada

AU - Carioti, Luca

AU - Gori, Caterina

AU - Forbici, Federica

AU - Salpini, Romina

AU - Vergori, Alessandra

AU - Gagliardini, Roberta

AU - Cicalini, Stefania

AU - Mondi, Annalisa

AU - Pinnetti, Carmela

AU - Mazzuti, Laura

AU - Turriziani, Ombretta

AU - Colafigli, Manuela

AU - Borghi, Vanni

AU - Montella, Francesco

AU - Pennica, Alfredo

AU - Lichtner, Miriam

AU - Girardi, Enrico

AU - Andreoni, Massimo

AU - Mussini, Cristina

AU - Antinori, Andrea

AU - Ceccherini-Silberstein, Francesca

AU - Perno, Carlo Federico

AU - Santoro, Maria Mercedes

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objective: We evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 in Italy. Methods: Phylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms and identify MTCs. MTCs were divided into small (SMTCs, 2-3 sequences), medium (MMTCs, 4-9 sequences) and large (LMTCs, ≥10 sequences). Factors associated with MTCs were evaluated using logistic regression analysis. Results: 145 MTCs were identified and involved 666 individuals (35.2%); 319 of them (16.9%) were included in 13 LMTCs, 111 (5.9%) in 20 MMTCs and 236 (12.5%) in 112 SMTCs. Compared with individuals out of MTCs, individuals involved in MTCs were prevalently Italian (72.7% vs 30.9%, p<0.001), male (82.9% vs 62.3%, p<0.001) and men who have sex with men (MSM) (43.5% vs 14.5%, p<0.001). Individuals in MTCs were also younger (median (IQR) years: 41 (35-49) vs 43 (36-51), p<0.001) and had higher CD4 cell count in comparison with individuals out of MTCs (median (IQR) cells/mm 3 : 10 9 /L 0.4 (0.265-0.587) vs 0.246 (0.082-0.417), p<0.001). The viral load remained stable between the two groups (median (IQR) log 10 copies/mL: 4.8 (4.2-5.5) vs 5.0 (4.3-5.5), p=0.87). Logistic regression confirmed that certain factors such as being MSM, of Italian origin, younger age and higher CD4 cell count were significantly associated with MTCs. Conclusions: Our findings show that HIV-1 newly diagnosed individuals infected with non-B subtypes are involved in several MTCs in Italy. These MTCs include mainly Italians and MSM and highlight the complex phenomenon characterising the HIV-1 spread. This is important especially in view of monitoring the HIV epidemic and guiding the public health response.

AB - Objective: We evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 in Italy. Methods: Phylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms and identify MTCs. MTCs were divided into small (SMTCs, 2-3 sequences), medium (MMTCs, 4-9 sequences) and large (LMTCs, ≥10 sequences). Factors associated with MTCs were evaluated using logistic regression analysis. Results: 145 MTCs were identified and involved 666 individuals (35.2%); 319 of them (16.9%) were included in 13 LMTCs, 111 (5.9%) in 20 MMTCs and 236 (12.5%) in 112 SMTCs. Compared with individuals out of MTCs, individuals involved in MTCs were prevalently Italian (72.7% vs 30.9%, p<0.001), male (82.9% vs 62.3%, p<0.001) and men who have sex with men (MSM) (43.5% vs 14.5%, p<0.001). Individuals in MTCs were also younger (median (IQR) years: 41 (35-49) vs 43 (36-51), p<0.001) and had higher CD4 cell count in comparison with individuals out of MTCs (median (IQR) cells/mm 3 : 10 9 /L 0.4 (0.265-0.587) vs 0.246 (0.082-0.417), p<0.001). The viral load remained stable between the two groups (median (IQR) log 10 copies/mL: 4.8 (4.2-5.5) vs 5.0 (4.3-5.5), p=0.87). Logistic regression confirmed that certain factors such as being MSM, of Italian origin, younger age and higher CD4 cell count were significantly associated with MTCs. Conclusions: Our findings show that HIV-1 newly diagnosed individuals infected with non-B subtypes are involved in several MTCs in Italy. These MTCs include mainly Italians and MSM and highlight the complex phenomenon characterising the HIV-1 spread. This is important especially in view of monitoring the HIV epidemic and guiding the public health response.

KW - HIV

KW - molecular epidemiology

KW - transmission dynamics

KW - transmission networks

KW - virology HIV

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U2 - 10.1136/sextrans-2019-054017

DO - 10.1136/sextrans-2019-054017

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JO - Sexually Transmitted Infections

JF - Sexually Transmitted Infections

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