Characterisation of the immune-related transcriptome in resected biliary tract cancers

Michele Ghidini, Michele Ghidini, Michele Ghidini, Luciano Cascione, Pietro Carotenuto, Andrea Lampis, Francesco Trevisani, Francesco Trevisani, Maria Chiara Previdi, Jens C. Hahne, Ian Said-Huntingford, Maya Raj, Alessandro Zerbi, Claudia Mescoli, Umberto Cillo, Massimo Rugge, Massimo Roncalli, Guido Torzilli, Lorenza Rimassa, Armando Santoro & 5 others Nicola Valeri, Nicola Valeri, Matteo Fassan, Chiara Braconi, Chiara Braconi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

© 2017 The Author(s) Although biliary tract cancers (BTCs) are known to have an inflammatory component, a detailed characterisation of immune-related transcripts has never been performed. In these studies, nCounter PanCancer Immune Profiling Panel was used to assess the expression of 770 immune-related transcripts in the tumour tissues (TTs) and matched adjacent tissues (ATs) of resected BTCs. Cox regression analysis and Kaplan–Meier methods were used to correlate findings with relapse-free survival (RFS). The first analysis in the TT and AT of an exploratory set (n = 22) showed deregulation of 39 transcripts associated with T-cell activation. Risk of recurrence was associated with a greater number of genes deregulated in AT in comparison to TT. Analysis in the whole set (n = 53) showed a correlation between AT cytotoxic T-lymphocyte antigen-4 (CTLA4) expression and RFS, which maintained statistical significance at multivariate analysis. CTLA4 expression correlated with forkhead box P3 (FOXP3) expression, suggesting enrichment in T regulatory cells. CTLA4 is known to act by binding to the cluster of differentiation 80 (CD80). No association was seen between AT CD80 expression and RFS. However, CD80 expression differentiated prognosis in patients who received adjuvant chemotherapy. We showed that the immunomodulatory transcriptome is deregulated in resected BTCs. Our study includes a small number of patients and does not enable to draw definitive conclusions; however, it provides useful insights into potential transcripts that may deserve further investigation in larger cohorts of patients. Transcript Profiling Nanostring data have been submitted to GEO repository: GSE906 98 and GSE90699.
Original languageEnglish
Pages (from-to)158-165
Number of pages8
JournalEuropean Journal of Cancer
Volume86
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Biliary Tract Neoplasms
Transcriptome
CTLA-4 Antigen
Recurrence
Survival
Neoplasms
Regulatory T-Lymphocytes
Adjuvant Chemotherapy
Multivariate Analysis
Regression Analysis
T-Lymphocytes

Keywords

  • Adjuvant
  • CD80
  • Cholangiocarcinoma
  • CTLA4
  • Treg

Cite this

Ghidini, M., Ghidini, M., Ghidini, M., Cascione, L., Carotenuto, P., Lampis, A., ... Braconi, C. (2017). Characterisation of the immune-related transcriptome in resected biliary tract cancers. European Journal of Cancer, 86, 158-165. https://doi.org/10.1016/j.ejca.2017.09.005

Characterisation of the immune-related transcriptome in resected biliary tract cancers. / Ghidini, Michele; Ghidini, Michele; Ghidini, Michele; Cascione, Luciano; Carotenuto, Pietro; Lampis, Andrea; Trevisani, Francesco; Trevisani, Francesco; Previdi, Maria Chiara; Hahne, Jens C.; Said-Huntingford, Ian; Raj, Maya; Zerbi, Alessandro; Mescoli, Claudia; Cillo, Umberto; Rugge, Massimo; Roncalli, Massimo; Torzilli, Guido; Rimassa, Lorenza; Santoro, Armando; Valeri, Nicola; Valeri, Nicola; Fassan, Matteo; Braconi, Chiara; Braconi, Chiara.

In: European Journal of Cancer, Vol. 86, 01.11.2017, p. 158-165.

Research output: Contribution to journalArticle

Ghidini, M, Ghidini, M, Ghidini, M, Cascione, L, Carotenuto, P, Lampis, A, Trevisani, F, Trevisani, F, Previdi, MC, Hahne, JC, Said-Huntingford, I, Raj, M, Zerbi, A, Mescoli, C, Cillo, U, Rugge, M, Roncalli, M, Torzilli, G, Rimassa, L, Santoro, A, Valeri, N, Valeri, N, Fassan, M, Braconi, C & Braconi, C 2017, 'Characterisation of the immune-related transcriptome in resected biliary tract cancers', European Journal of Cancer, vol. 86, pp. 158-165. https://doi.org/10.1016/j.ejca.2017.09.005
Ghidini, Michele ; Ghidini, Michele ; Ghidini, Michele ; Cascione, Luciano ; Carotenuto, Pietro ; Lampis, Andrea ; Trevisani, Francesco ; Trevisani, Francesco ; Previdi, Maria Chiara ; Hahne, Jens C. ; Said-Huntingford, Ian ; Raj, Maya ; Zerbi, Alessandro ; Mescoli, Claudia ; Cillo, Umberto ; Rugge, Massimo ; Roncalli, Massimo ; Torzilli, Guido ; Rimassa, Lorenza ; Santoro, Armando ; Valeri, Nicola ; Valeri, Nicola ; Fassan, Matteo ; Braconi, Chiara ; Braconi, Chiara. / Characterisation of the immune-related transcriptome in resected biliary tract cancers. In: European Journal of Cancer. 2017 ; Vol. 86. pp. 158-165.
@article{689c88f95ac345c7980ef6e564ea0fa5,
title = "Characterisation of the immune-related transcriptome in resected biliary tract cancers",
abstract = "{\circledC} 2017 The Author(s) Although biliary tract cancers (BTCs) are known to have an inflammatory component, a detailed characterisation of immune-related transcripts has never been performed. In these studies, nCounter PanCancer Immune Profiling Panel was used to assess the expression of 770 immune-related transcripts in the tumour tissues (TTs) and matched adjacent tissues (ATs) of resected BTCs. Cox regression analysis and Kaplan–Meier methods were used to correlate findings with relapse-free survival (RFS). The first analysis in the TT and AT of an exploratory set (n = 22) showed deregulation of 39 transcripts associated with T-cell activation. Risk of recurrence was associated with a greater number of genes deregulated in AT in comparison to TT. Analysis in the whole set (n = 53) showed a correlation between AT cytotoxic T-lymphocyte antigen-4 (CTLA4) expression and RFS, which maintained statistical significance at multivariate analysis. CTLA4 expression correlated with forkhead box P3 (FOXP3) expression, suggesting enrichment in T regulatory cells. CTLA4 is known to act by binding to the cluster of differentiation 80 (CD80). No association was seen between AT CD80 expression and RFS. However, CD80 expression differentiated prognosis in patients who received adjuvant chemotherapy. We showed that the immunomodulatory transcriptome is deregulated in resected BTCs. Our study includes a small number of patients and does not enable to draw definitive conclusions; however, it provides useful insights into potential transcripts that may deserve further investigation in larger cohorts of patients. Transcript Profiling Nanostring data have been submitted to GEO repository: GSE906 98 and GSE90699.",
keywords = "Adjuvant, CD80, Cholangiocarcinoma, CTLA4, Treg",
author = "Michele Ghidini and Michele Ghidini and Michele Ghidini and Luciano Cascione and Pietro Carotenuto and Andrea Lampis and Francesco Trevisani and Francesco Trevisani and Previdi, {Maria Chiara} and Hahne, {Jens C.} and Ian Said-Huntingford and Maya Raj and Alessandro Zerbi and Claudia Mescoli and Umberto Cillo and Massimo Rugge and Massimo Roncalli and Guido Torzilli and Lorenza Rimassa and Armando Santoro and Nicola Valeri and Nicola Valeri and Matteo Fassan and Chiara Braconi and Chiara Braconi",
year = "2017",
month = "11",
day = "1",
doi = "10.1016/j.ejca.2017.09.005",
language = "English",
volume = "86",
pages = "158--165",
journal = "European Journal of Cancer",
issn = "0959-8049",
publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Characterisation of the immune-related transcriptome in resected biliary tract cancers

AU - Ghidini, Michele

AU - Ghidini, Michele

AU - Ghidini, Michele

AU - Cascione, Luciano

AU - Carotenuto, Pietro

AU - Lampis, Andrea

AU - Trevisani, Francesco

AU - Trevisani, Francesco

AU - Previdi, Maria Chiara

AU - Hahne, Jens C.

AU - Said-Huntingford, Ian

AU - Raj, Maya

AU - Zerbi, Alessandro

AU - Mescoli, Claudia

AU - Cillo, Umberto

AU - Rugge, Massimo

AU - Roncalli, Massimo

AU - Torzilli, Guido

AU - Rimassa, Lorenza

AU - Santoro, Armando

AU - Valeri, Nicola

AU - Valeri, Nicola

AU - Fassan, Matteo

AU - Braconi, Chiara

AU - Braconi, Chiara

PY - 2017/11/1

Y1 - 2017/11/1

N2 - © 2017 The Author(s) Although biliary tract cancers (BTCs) are known to have an inflammatory component, a detailed characterisation of immune-related transcripts has never been performed. In these studies, nCounter PanCancer Immune Profiling Panel was used to assess the expression of 770 immune-related transcripts in the tumour tissues (TTs) and matched adjacent tissues (ATs) of resected BTCs. Cox regression analysis and Kaplan–Meier methods were used to correlate findings with relapse-free survival (RFS). The first analysis in the TT and AT of an exploratory set (n = 22) showed deregulation of 39 transcripts associated with T-cell activation. Risk of recurrence was associated with a greater number of genes deregulated in AT in comparison to TT. Analysis in the whole set (n = 53) showed a correlation between AT cytotoxic T-lymphocyte antigen-4 (CTLA4) expression and RFS, which maintained statistical significance at multivariate analysis. CTLA4 expression correlated with forkhead box P3 (FOXP3) expression, suggesting enrichment in T regulatory cells. CTLA4 is known to act by binding to the cluster of differentiation 80 (CD80). No association was seen between AT CD80 expression and RFS. However, CD80 expression differentiated prognosis in patients who received adjuvant chemotherapy. We showed that the immunomodulatory transcriptome is deregulated in resected BTCs. Our study includes a small number of patients and does not enable to draw definitive conclusions; however, it provides useful insights into potential transcripts that may deserve further investigation in larger cohorts of patients. Transcript Profiling Nanostring data have been submitted to GEO repository: GSE906 98 and GSE90699.

AB - © 2017 The Author(s) Although biliary tract cancers (BTCs) are known to have an inflammatory component, a detailed characterisation of immune-related transcripts has never been performed. In these studies, nCounter PanCancer Immune Profiling Panel was used to assess the expression of 770 immune-related transcripts in the tumour tissues (TTs) and matched adjacent tissues (ATs) of resected BTCs. Cox regression analysis and Kaplan–Meier methods were used to correlate findings with relapse-free survival (RFS). The first analysis in the TT and AT of an exploratory set (n = 22) showed deregulation of 39 transcripts associated with T-cell activation. Risk of recurrence was associated with a greater number of genes deregulated in AT in comparison to TT. Analysis in the whole set (n = 53) showed a correlation between AT cytotoxic T-lymphocyte antigen-4 (CTLA4) expression and RFS, which maintained statistical significance at multivariate analysis. CTLA4 expression correlated with forkhead box P3 (FOXP3) expression, suggesting enrichment in T regulatory cells. CTLA4 is known to act by binding to the cluster of differentiation 80 (CD80). No association was seen between AT CD80 expression and RFS. However, CD80 expression differentiated prognosis in patients who received adjuvant chemotherapy. We showed that the immunomodulatory transcriptome is deregulated in resected BTCs. Our study includes a small number of patients and does not enable to draw definitive conclusions; however, it provides useful insights into potential transcripts that may deserve further investigation in larger cohorts of patients. Transcript Profiling Nanostring data have been submitted to GEO repository: GSE906 98 and GSE90699.

KW - Adjuvant

KW - CD80

KW - Cholangiocarcinoma

KW - CTLA4

KW - Treg

UR - http://www.scopus.com/inward/record.url?scp=85030567436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030567436&partnerID=8YFLogxK

U2 - 10.1016/j.ejca.2017.09.005

DO - 10.1016/j.ejca.2017.09.005

M3 - Article

VL - 86

SP - 158

EP - 165

JO - European Journal of Cancer

JF - European Journal of Cancer

SN - 0959-8049

ER -