Characteristics and their clinical relevance of respiratory syncytial virus types and genotypes circulating in Northern Italy in five consecutive winter seasons

Susanna Esposito, Antonio Piralla, Alberto Zampiero, Sonia Bianchini, Giada Di Pietro, Alessia Scala, Raffaella Pinzani, Emilio Fossali, Fausto Baldanti, Nicola Principi

Research output: Contribution to journalArticle

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Abstract

In order to investigate the genetic diversity and patterns of the co-circulating genotypes of respiratory syncytial virus (RSV) and their possible relationships with the severity of RSV infection, we studied all of the RSV-positive nasopharyngeal samples collected from children during five consecutive winters (2009-2010, 2010-2011, 2011-2012, 2012-2013 and 2013-2014). The RSVs were detected using the respiratory virus panel fast assay and single-tube RT-PCR, their nucleotides were sequenced, and they were tested for positive selection. Of the 165 positive samples, 131 (79.4%) carried RSV-A and 34 (20.6%) RSV-B; both groups co-circulated in all of the study periods, with RSV-A predominating in all the seasons except for winter 2010-2011, which had a predominance of RSV-B. Phylogenetic analysis of the RSV-A sequences identified genotypes NA1 and ON1, the second replacing the first during the last two years of the study period. The RSV-B belonged to genotypes BA9 and BA10. BA9 was detected in all the years of the study whereas BA only desultorily. Comparison of the subjects infected by RSV-A and RSV-B types did not reveal any significant differences, but the children infected by genotype A/NA1 more frequently had lower respiratory tract infections (p

Original languageEnglish
Article numbere0129369
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 5 2015

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Respiratory Syncytial Viruses
Viruses
Italy
Genotype
viruses
winter
genotype
Macacine herpesvirus 1
Respiratory Syncytial Virus Infections
respiratory tract diseases
Respiratory Tract Infections
nucleotides
reverse transcriptase polymerase chain reaction
Nucleotides
sampling
Assays
genetic variation
Polymerase Chain Reaction
phylogeny
assays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Characteristics and their clinical relevance of respiratory syncytial virus types and genotypes circulating in Northern Italy in five consecutive winter seasons. / Esposito, Susanna; Piralla, Antonio; Zampiero, Alberto; Bianchini, Sonia; Di Pietro, Giada; Scala, Alessia; Pinzani, Raffaella; Fossali, Emilio; Baldanti, Fausto; Principi, Nicola.

In: PLoS One, Vol. 10, No. 6, e0129369, 05.06.2015.

Research output: Contribution to journalArticle

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AU - Scala, Alessia

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