Characterization and Distribution of Protein Kinase C Isoforms in Human Skin Fibroblasts

M. Racchi, S. Bergamaschi, S. Govoni, W. C. Wetsel, A. Bianchetti, G. Binetti, F. Battaini, M. Trabucchi

Research output: Contribution to journalArticle

Abstract

In the present study we have examined the distribution of several isoforms of protein kinase C, a lipid-regulated serine/threonine kinase essential for signal transduction and cell regulation, in cultured human skin fibroblasts. By Western blot analysis we have detected the presence of at least three of the known protein kinase C isoforms. The calcium-dependent protein kinase Cα was primarily associated with the cytosolic fraction. Three non-calcium-dependent isoforms, protein kinases Cε{lunate}, Cδ, and Cζ, were also detected. Protein kinases Cζ and Cδ were present primarily in the cytosol, while protein kinase Cε{lunate} was associated primarily with the membrane fraction. Binding and activity studies were consistent with the pattern of expression and distribution defined by Western blot analysis. These results provide a useful frame of reference for the study of isoform-specific effects of protein kinase C in the regulation of cell growth and metabolism.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume314
Issue number1
DOIs
Publication statusPublished - Oct 1994

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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    Racchi, M., Bergamaschi, S., Govoni, S., Wetsel, W. C., Bianchetti, A., Binetti, G., Battaini, F., & Trabucchi, M. (1994). Characterization and Distribution of Protein Kinase C Isoforms in Human Skin Fibroblasts. Archives of Biochemistry and Biophysics, 314(1), 107-111. https://doi.org/10.1006/abbi.1994.1417