Abstract
Endonuclease G (Endo G) is a nuclease of prokaryotic lineage found in the mitochondria of vertebrates that has been suggested to play a role in mitochondrial DNA (mtDNA) replication. We have isolated and sequenced the entire mouse endo G gene, determined the limits of the mRNA, and mapped the promoter region. The coding sequence of the single copy gene is interrupted by two introns and analysis of the transcripts does not support a model by which more than one Endo G isoform could be produced by alternative splicing. We have also characterized a full-length human Endo G cDNA and comparison at the protein level of the human, bovine, and murine nucleases indicates a high degree of conservation except in the respective mitochondrial targeting signals. Endo G is ubiquitously expressed and the steady-state levels of its mRNA vary by a factor greater than seven between different tissues. The relationship between the mtDNA copy number and Endo G mRNA levels is not strictly proportional but tissues richer in mtDNA have higher amounts of the mRNA and vice versa.
| Original language | English |
|---|---|
| Pages (from-to) | 1111-1122 |
| Number of pages | 12 |
| Journal | DNA and Cell Biology |
| Volume | 16 |
| Issue number | 9 |
| Publication status | Published - 1997 |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology
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Characterization and expression of the mouse Endonuclease G gene. / Prats, Eva; Noël, Micheline; Létourneau, Julie; Tiranti, Valeria; Vaqué, Jaume; Debón, Rosa; Zeviani, Massimo; Cornudella, Lluís; Ruiz-Carrillo, Adolf.
In: DNA and Cell Biology, Vol. 16, No. 9, 1997, p. 1111-1122.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization and expression of the mouse Endonuclease G gene
AU - Prats, Eva
AU - Noël, Micheline
AU - Létourneau, Julie
AU - Tiranti, Valeria
AU - Vaqué, Jaume
AU - Debón, Rosa
AU - Zeviani, Massimo
AU - Cornudella, Lluís
AU - Ruiz-Carrillo, Adolf
PY - 1997
Y1 - 1997
N2 - Endonuclease G (Endo G) is a nuclease of prokaryotic lineage found in the mitochondria of vertebrates that has been suggested to play a role in mitochondrial DNA (mtDNA) replication. We have isolated and sequenced the entire mouse endo G gene, determined the limits of the mRNA, and mapped the promoter region. The coding sequence of the single copy gene is interrupted by two introns and analysis of the transcripts does not support a model by which more than one Endo G isoform could be produced by alternative splicing. We have also characterized a full-length human Endo G cDNA and comparison at the protein level of the human, bovine, and murine nucleases indicates a high degree of conservation except in the respective mitochondrial targeting signals. Endo G is ubiquitously expressed and the steady-state levels of its mRNA vary by a factor greater than seven between different tissues. The relationship between the mtDNA copy number and Endo G mRNA levels is not strictly proportional but tissues richer in mtDNA have higher amounts of the mRNA and vice versa.
AB - Endonuclease G (Endo G) is a nuclease of prokaryotic lineage found in the mitochondria of vertebrates that has been suggested to play a role in mitochondrial DNA (mtDNA) replication. We have isolated and sequenced the entire mouse endo G gene, determined the limits of the mRNA, and mapped the promoter region. The coding sequence of the single copy gene is interrupted by two introns and analysis of the transcripts does not support a model by which more than one Endo G isoform could be produced by alternative splicing. We have also characterized a full-length human Endo G cDNA and comparison at the protein level of the human, bovine, and murine nucleases indicates a high degree of conservation except in the respective mitochondrial targeting signals. Endo G is ubiquitously expressed and the steady-state levels of its mRNA vary by a factor greater than seven between different tissues. The relationship between the mtDNA copy number and Endo G mRNA levels is not strictly proportional but tissues richer in mtDNA have higher amounts of the mRNA and vice versa.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031405183&partnerID=8YFLogxK
M3 - Article
C2 - 9324313
AN - SCOPUS:0031405183
VL - 16
SP - 1111
EP - 1122
JO - DNA and Cell Biology
JF - DNA and Cell Biology
SN - 1044-5498
IS - 9
ER -