Characterization by somatic cell genetics of a monoclonal antibody to the MDR1 gene product (P-glycoprotein): Determination of P-glycoprotein expression in multi-drug-resistant KB and CEM cell variants

C. Cenciarelli, S. J. Currier, M. C. Willingham, F. Thiebaut, U. A. Germann, A. V. Rutherford, M. M. Gottesman, S. Barca, M. Tombesi, S. Morrone, A. Santoni, M. Mariani, C. Ramoni, M. L. Dupuis, M. Cianfriglia

Research output: Contribution to journalArticlepeer-review

Abstract

We isolated an IgG(2a) murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi-drug-resistant (MDR) human cells. Its specificity toward the MDR1 gene product (P-glycoprotein) has been demonstrated by the concordant segregation of the MAb57 epitope with the MDR1 gene in interspecific mouse x human cell hybrids, and the reactivity of several different MDR1 gene-expressing cells with MAb57, particularly insect cells acutely infected with a baculovirus encoding the MDR1 gene. MAb57 can be used to detect, by flow cytometry, variations in the relative drug-resistance levels of several MDR KB and CEM cell variants. This immunological probe has also proven useful in selectively destroying MDR target cells in an antibody-dependent cell-mediated (ADCC) assay system as well as in detecting P-glycoprotein expression in normal and malignant tissues and cells.

Original languageEnglish
Pages (from-to)533-543
Number of pages11
JournalInternational Journal of Cancer
Volume47
Issue number4
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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