Characterization of 17 strains belonging to the mycobacterium simiae complex and description of mycobacterium paraense sp. Nov.

Ana R. Fusco Da Costa; Tarcisio Fedrizzi; Maria L. Lopes; Monica Pecorari; Wana L. Oliveira Da Costa; Elisabetta Giacobazzi; Jeann R. Da Costa Bahia; Veronica De Sanctis; Karla V. Batista Lima; Roberto Bertorelli; Antonella Grottola; Anna Fabio; Alessandro Mariottini; Pamela Ferretti; Francesca Di Leva; Giulia Fregni Serpini; Sara Tagliazucchi; Fabio Rumpianesi; Olivier Jousson; Nicola Segata; Enrico Tortoli

doi:10.1099/ijs.0.068395-0

Characterization of 17 strains belonging to the mycobacterium simiae complex and description of mycobacterium paraense sp. Nov.

Ana R. Fusco Da Costa, Tarcisio Fedrizzi, Maria L. Lopes, Monica Pecorari, Wana L. Oliveira Da Costa, Elisabetta Giacobazzi, Jeann R. Da Costa Bahia, Veronica De Sanctis, Karla V. Batista Lima, Roberto Bertorelli, Antonella Grottola, Anna Fabio, Alessandro Mariottini, Pamela Ferretti, Francesca Di Leva, Giulia Fregni Serpini, Sara Tagliazucchi, Fabio Rumpianesi, Olivier Jousson, Nicola SegataEnrico Tortoli

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Fourteen mycobacterial strains isolated from pulmonary samples of independent patients in the state of Para´ (Brazil), and three strains isolated in Italy, were characterized using a polyphasic approach. Thorough genetic investigation, including whole-genome sequencing, demonstrated that the strains belong to the M. simiae complex, being most closely related to Mycobacterium interjectum. For 14 of the strains, evidence emerged supporting their inclusion in a previously unreported species of the genus Mycobacterium, for which the name Mycobacterium paraense sp. nov. is proposed (type strain, IEC26^T5DSM 46749^T5CCUG 66121^T). The novel species is characterized by slow growth, unpigmented or pale yellow scotochromogenic colonies, and a HPLC mycolic acid profile different from other known mycobacteria. In different genetic regions, high sequence microheterogeneity was detected.

Original language	English
Pages (from-to)	656-662
Number of pages	7
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	65
Issue number	2
DOIs	https://doi.org/10.1099/ijs.0.068395-0
Publication status	Published - 2015

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics
Medicine(all)

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Fusco Da Costa, A. R., Fedrizzi, T., Lopes, M. L., Pecorari, M., Oliveira Da Costa, W. L., Giacobazzi, E., Da Costa Bahia, J. R., De Sanctis, V., Batista Lima, K. V., Bertorelli, R., Grottola, A., Fabio, A., Mariottini, A., Ferretti, P., Di Leva, F., Serpini, G. F., Tagliazucchi, S., Rumpianesi, F., Jousson, O., ... Tortoli, E. (2015). Characterization of 17 strains belonging to the mycobacterium simiae complex and description of mycobacterium paraense sp. Nov. International Journal of Systematic and Evolutionary Microbiology, 65(2), 656-662. https://doi.org/10.1099/ijs.0.068395-0

Characterization of 17 strains belonging to the mycobacterium simiae complex and description of mycobacterium paraense sp. Nov. / Fusco Da Costa, Ana R.; Fedrizzi, Tarcisio; Lopes, Maria L.; Pecorari, Monica; Oliveira Da Costa, Wana L.; Giacobazzi, Elisabetta; Da Costa Bahia, Jeann R.; De Sanctis, Veronica; Batista Lima, Karla V.; Bertorelli, Roberto; Grottola, Antonella; Fabio, Anna; Mariottini, Alessandro; Ferretti, Pamela; Di Leva, Francesca; Serpini, Giulia Fregni; Tagliazucchi, Sara; Rumpianesi, Fabio; Jousson, Olivier; Segata, Nicola; Tortoli, Enrico.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 2, 2015, p. 656-662.

Research output: Contribution to journal › Article › peer-review

Fusco Da Costa, AR, Fedrizzi, T, Lopes, ML, Pecorari, M, Oliveira Da Costa, WL, Giacobazzi, E, Da Costa Bahia, JR, De Sanctis, V, Batista Lima, KV, Bertorelli, R, Grottola, A, Fabio, A, Mariottini, A, Ferretti, P, Di Leva, F, Serpini, GF, Tagliazucchi, S, Rumpianesi, F, Jousson, O, Segata, N & Tortoli, E 2015, 'Characterization of 17 strains belonging to the mycobacterium simiae complex and description of mycobacterium paraense sp. Nov.', International Journal of Systematic and Evolutionary Microbiology, vol. 65, no. 2, pp. 656-662. https://doi.org/10.1099/ijs.0.068395-0

Fusco Da Costa, Ana R. ; Fedrizzi, Tarcisio ; Lopes, Maria L. ; Pecorari, Monica ; Oliveira Da Costa, Wana L. ; Giacobazzi, Elisabetta ; Da Costa Bahia, Jeann R. ; De Sanctis, Veronica ; Batista Lima, Karla V. ; Bertorelli, Roberto ; Grottola, Antonella ; Fabio, Anna ; Mariottini, Alessandro ; Ferretti, Pamela ; Di Leva, Francesca ; Serpini, Giulia Fregni ; Tagliazucchi, Sara ; Rumpianesi, Fabio ; Jousson, Olivier ; Segata, Nicola ; Tortoli, Enrico. / Characterization of 17 strains belonging to the mycobacterium simiae complex and description of mycobacterium paraense sp. Nov. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 2. pp. 656-662.

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abstract = "Fourteen mycobacterial strains isolated from pulmonary samples of independent patients in the state of Para´ (Brazil), and three strains isolated in Italy, were characterized using a polyphasic approach. Thorough genetic investigation, including whole-genome sequencing, demonstrated that the strains belong to the M. simiae complex, being most closely related to Mycobacterium interjectum. For 14 of the strains, evidence emerged supporting their inclusion in a previously unreported species of the genus Mycobacterium, for which the name Mycobacterium paraense sp. nov. is proposed (type strain, IEC26T5DSM 46749T5CCUG 66121T). The novel species is characterized by slow growth, unpigmented or pale yellow scotochromogenic colonies, and a HPLC mycolic acid profile different from other known mycobacteria. In different genetic regions, high sequence microheterogeneity was detected.",

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AU - Pecorari, Monica

AU - Oliveira Da Costa, Wana L.

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AU - Da Costa Bahia, Jeann R.

AU - De Sanctis, Veronica

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AU - Grottola, Antonella

AU - Fabio, Anna

AU - Mariottini, Alessandro

AU - Ferretti, Pamela

AU - Di Leva, Francesca

AU - Serpini, Giulia Fregni

AU - Tagliazucchi, Sara

AU - Rumpianesi, Fabio

AU - Jousson, Olivier

AU - Segata, Nicola

AU - Tortoli, Enrico

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