Characterization of a Chemoattractant for Endothelium Induced by Angiogenesis Effectors

Katari S. Raju, Giulio Alessandri, Pietro M. Gullino

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The mechanism of neovascularization was further explored by the use of chemically defined angiogenesis effectors. The vascularization of the rabbit cornea was selected as an experimental approach that permits comparison of one cornea treated by the angiogenesis effector with the contralateral cornea of the same subject treated by the same molecule deprived of angiogenic capacity. Under these conditions, we observed that neovascularization was initiated by the appearance of a chemoattractant for the bovine capillary endothelium only in the cornea treated by the angiogenesis effector. The chemoattractant was purified about 150-fold by a single-step procedure, using geiatin:Sepharose affinity chromatography. Chemoattraction resulted from the combined effect of a chemotactic factors) and an activating factors). The association of the two enhanced 5- to 8-fold the motility of the capillary endothelium in a concentration-dependent manner with optimum at 0.2 mg/ml. The activating factors) does not have chemotactic capacity, but without it, Chemotaxis is reduced to about one half. The chemotactic complex was present in the cornea regardless of the nature of the angiogenesis effector used as the triggering device. Heat and proteases eliminated chemotaxis and destroyed the chemotactic complex. Thus, neovascularization may be triggered by effectors able to induce in the cornea proteins, normally not present, that influence angiogenesis via mobilization of capillary endothelium.

Original languageEnglish
Pages (from-to)1579-1584
Number of pages6
JournalCancer Research
Issue number4
Publication statusPublished - Apr 1 1984

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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