Characterization of a cyclosporin A-sensitive activation pathway in cultured T and natural killer cells

C. Cantoni, A. Cambiaggi, S. Sforzini, A. Poggi, M. Viale, R. Biassoni, S. Ferrini

Research output: Contribution to journalArticle

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Abstract

Previously, the authors have described a molecule, identified by the LD6 monoclonal antibody (MoAb), present at the cell surface of long-term cultured T and Natural Killer (NK) cells which is involved in cell triggering. In the study described here the authors used biotin surface labelling and immunoprecipitation to show that LD6 MoAb recognizes a surface protein of approximately 65 kDa. In combination with submitogenic concentrations of phorbol esters (PMA), LD6 MoAb was able to induce accumulation of mRNA specific for GM-CSF, γ-IFN and TNF-α and release of these cytokines by LD6+ T-cell lines. Both lymphokine production and lymphokine-specific mRNA accumulation induced by the LD6 MoAb were blocked totally by Cyclosporin A (CsA). To investigate the mechanism(s) of signal transduction through this activatory pathway, the authors performed Ca++ mobilization experiments. The results of these experiments suggested a role for Ca++ in signal transduction. The Ca++ mobilization induced by LD6 MoAb cross-linking could be inhibited totally by the use of pertussis toxin, indicating a possible role for G proteins in signalling through the LD6 MoAb-reactive molecule. Western blot analysis performed with an anti-phosphotyrosine antibody did not suggest that tyrosine kinase activation has a role.

Original languageEnglish
Pages (from-to)373-379
Number of pages7
JournalScandinavian Journal of Immunology
Volume39
Issue number4
DOIs
Publication statusPublished - 1994

Fingerprint

Natural Killer T-Cells
Cyclosporine
Monoclonal Antibodies
Lymphokines
Signal Transduction
Messenger RNA
Phosphotyrosine
Pertussis Toxin
Phorbol Esters
Granulocyte-Macrophage Colony-Stimulating Factor
Biotin
GTP-Binding Proteins
Immunoprecipitation
Protein-Tyrosine Kinases
Anti-Idiotypic Antibodies
Membrane Proteins
Western Blotting
Cytokines
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of a cyclosporin A-sensitive activation pathway in cultured T and natural killer cells. / Cantoni, C.; Cambiaggi, A.; Sforzini, S.; Poggi, A.; Viale, M.; Biassoni, R.; Ferrini, S.

In: Scandinavian Journal of Immunology, Vol. 39, No. 4, 1994, p. 373-379.

Research output: Contribution to journalArticle

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AU - Cantoni, C.

AU - Cambiaggi, A.

AU - Sforzini, S.

AU - Poggi, A.

AU - Viale, M.

AU - Biassoni, R.

AU - Ferrini, S.

PY - 1994

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