TY - JOUR
T1 - Characterization of a Distal 5′-Flanking Region (-2010/-630) of Human GM-CSF
AU - Fiorentini, P.
AU - Musso, M.
AU - Penco, S.
AU - Giuffrida, R.
AU - Pistoia, V.
AU - Garre, C.
AU - Bianchiscarra, G.
PY - 1995/9/25
Y1 - 1995/9/25
N2 - The 5′-flanking region of the human GM-CSF gene was subcloned from the phagic clone λJ1-16 to detect cis-elements involved in GM-CSF constitutive expression. We determined and sequenced an uncharacterized promoter region of 1381 bp (-2010 to -630), named pPF2000. Putative binding sites of several transcriptional factors were found. Progressive deletion mutants of the PF2000 were analyzed by measuring the linked CAT activities, in constitutive (5637) and inducibile (PEU) GM-CSF-producing cells. A positive distal sequence (268 bp), between -2010 and -1742, responsible for the high constitutive expression of GM-CSF in 5637 carcinoma cell line was found.
AB - The 5′-flanking region of the human GM-CSF gene was subcloned from the phagic clone λJ1-16 to detect cis-elements involved in GM-CSF constitutive expression. We determined and sequenced an uncharacterized promoter region of 1381 bp (-2010 to -630), named pPF2000. Putative binding sites of several transcriptional factors were found. Progressive deletion mutants of the PF2000 were analyzed by measuring the linked CAT activities, in constitutive (5637) and inducibile (PEU) GM-CSF-producing cells. A positive distal sequence (268 bp), between -2010 and -1742, responsible for the high constitutive expression of GM-CSF in 5637 carcinoma cell line was found.
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U2 - 10.1006/bbrc.1995.2387
DO - 10.1006/bbrc.1995.2387
M3 - Article
C2 - 7575504
AN - SCOPUS:0028788979
VL - 214
SP - 1015
EP - 1022
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -