Characterization of a murine gene homologous to the bovine CaCC chloride channel

L. Romio, L. Musante, R. Cinti, M. Seri, O. Moran, O. Zegarra-Moran, L. J V Galietta

Research output: Contribution to journalArticle

Abstract

The bovine CaCC protein is a putative Ca2+-dependent Cl- channel of airway epithelial cells. Therefore, CaCC proteins could contribute to transepithelial Cl- transport and accordingly modify the phenotype of cystic fibrosis (CF) patients. We have identified a murine EST containing a full- length cDNA coding for a 902-amino-acid protein highly homologous to bovine CaCC. The murine gene (mCaCC) maps to chromosome 3 at the H2-H3 band and is expressed, as indicated by Northern blot analysis, in mouse skin and kidney but not in brain, heart, lung or testis. RT-PCR indicates a low expression in tracheal epithelial cells. Heterologous expression of mCaCC in Xenopus oocytes elicits membrane currents that are anion-selective and inhibited by DIDS and by niflumic acid, a blocker of the endogenous chloride current in oocytes. The identification of genes belonging to the CaCC family will help to evaluate their role as ion channels or channel regulators and their actual contribution to epithelial chloride transport.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalGene
Volume228
Issue number1-2
DOIs
Publication statusPublished - Mar 4 1999

Keywords

  • Airway epithelium
  • Cell adhesion molecule
  • Chloride secretion
  • Cystic fibrosis

ASJC Scopus subject areas

  • Genetics

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    Romio, L., Musante, L., Cinti, R., Seri, M., Moran, O., Zegarra-Moran, O., & Galietta, L. J. V. (1999). Characterization of a murine gene homologous to the bovine CaCC chloride channel. Gene, 228(1-2), 181-188. https://doi.org/10.1016/S0378-1119(98)00620-9