Characterization of a murine gene homologous to the bovine CaCC chloride channel

L. Romio, L. Musante, R. Cinti, M. Seri, O. Moran, O. Zegarra-Moran, L. J V Galietta

Research output: Contribution to journalArticle

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Abstract

The bovine CaCC protein is a putative Ca2+-dependent Cl- channel of airway epithelial cells. Therefore, CaCC proteins could contribute to transepithelial Cl- transport and accordingly modify the phenotype of cystic fibrosis (CF) patients. We have identified a murine EST containing a full- length cDNA coding for a 902-amino-acid protein highly homologous to bovine CaCC. The murine gene (mCaCC) maps to chromosome 3 at the H2-H3 band and is expressed, as indicated by Northern blot analysis, in mouse skin and kidney but not in brain, heart, lung or testis. RT-PCR indicates a low expression in tracheal epithelial cells. Heterologous expression of mCaCC in Xenopus oocytes elicits membrane currents that are anion-selective and inhibited by DIDS and by niflumic acid, a blocker of the endogenous chloride current in oocytes. The identification of genes belonging to the CaCC family will help to evaluate their role as ion channels or channel regulators and their actual contribution to epithelial chloride transport.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalGene
Volume228
Issue number1-2
DOIs
Publication statusPublished - Mar 4 1999

Fingerprint

Chloride Channels
Oocytes
Chlorides
Epithelial Cells
Niflumic Acid
Genes
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Proteins
Chromosomes, Human, Pair 3
Expressed Sequence Tags
Xenopus
Ion Channels
Cystic Fibrosis
Northern Blotting
Anions
Testis
Complementary DNA
Phenotype
Kidney
Amino Acids

Keywords

  • Airway epithelium
  • Cell adhesion molecule
  • Chloride secretion
  • Cystic fibrosis

ASJC Scopus subject areas

  • Genetics

Cite this

Romio, L., Musante, L., Cinti, R., Seri, M., Moran, O., Zegarra-Moran, O., & Galietta, L. J. V. (1999). Characterization of a murine gene homologous to the bovine CaCC chloride channel. Gene, 228(1-2), 181-188. https://doi.org/10.1016/S0378-1119(98)00620-9

Characterization of a murine gene homologous to the bovine CaCC chloride channel. / Romio, L.; Musante, L.; Cinti, R.; Seri, M.; Moran, O.; Zegarra-Moran, O.; Galietta, L. J V.

In: Gene, Vol. 228, No. 1-2, 04.03.1999, p. 181-188.

Research output: Contribution to journalArticle

Romio, L, Musante, L, Cinti, R, Seri, M, Moran, O, Zegarra-Moran, O & Galietta, LJV 1999, 'Characterization of a murine gene homologous to the bovine CaCC chloride channel', Gene, vol. 228, no. 1-2, pp. 181-188. https://doi.org/10.1016/S0378-1119(98)00620-9
Romio L, Musante L, Cinti R, Seri M, Moran O, Zegarra-Moran O et al. Characterization of a murine gene homologous to the bovine CaCC chloride channel. Gene. 1999 Mar 4;228(1-2):181-188. https://doi.org/10.1016/S0378-1119(98)00620-9
Romio, L. ; Musante, L. ; Cinti, R. ; Seri, M. ; Moran, O. ; Zegarra-Moran, O. ; Galietta, L. J V. / Characterization of a murine gene homologous to the bovine CaCC chloride channel. In: Gene. 1999 ; Vol. 228, No. 1-2. pp. 181-188.
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