Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.)

Lara Padovan, Ludovica Segat, Alessandro Tossi, Tercilio Calsa, Akio Kido Ederson, Lucas Brandao, Rafael L. Guimarães, Valesca Pandolfi, Maria Clara Pestana-Calsa, Luís Carlos Belarmino, Ana Maria Benko-Iseppon, Sergio Crovella

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna un-guiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalProtein and Peptide Letters
Volume17
Issue number3
DOIs
Publication statusPublished - 2010

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Defensins
Genes
Vigna
Databases
Polymerase Chain Reaction
DNA

Keywords

  • Antimicrobial peptides
  • Cowpea mosaic virus
  • Evolution
  • Plant defensin
  • Protein function
  • Vigna unguiculata

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology

Cite this

Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.). / Padovan, Lara; Segat, Ludovica; Tossi, Alessandro; Calsa, Tercilio; Ederson, Akio Kido; Brandao, Lucas; Guimarães, Rafael L.; Pandolfi, Valesca; Pestana-Calsa, Maria Clara; Belarmino, Luís Carlos; Benko-Iseppon, Ana Maria; Crovella, Sergio.

In: Protein and Peptide Letters, Vol. 17, No. 3, 2010, p. 297-304.

Research output: Contribution to journalArticle

Padovan, L, Segat, L, Tossi, A, Calsa, T, Ederson, AK, Brandao, L, Guimarães, RL, Pandolfi, V, Pestana-Calsa, MC, Belarmino, LC, Benko-Iseppon, AM & Crovella, S 2010, 'Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.)', Protein and Peptide Letters, vol. 17, no. 3, pp. 297-304. https://doi.org/10.2174/092986610790780350
Padovan, Lara ; Segat, Ludovica ; Tossi, Alessandro ; Calsa, Tercilio ; Ederson, Akio Kido ; Brandao, Lucas ; Guimarães, Rafael L. ; Pandolfi, Valesca ; Pestana-Calsa, Maria Clara ; Belarmino, Luís Carlos ; Benko-Iseppon, Ana Maria ; Crovella, Sergio. / Characterization of a new defensin from cowpea (Vigna unguiculata (L.) Walp.). In: Protein and Peptide Letters. 2010 ; Vol. 17, No. 3. pp. 297-304.
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