Characterization of a new high-temperature-induced 66-kDa heat-shock protein, antigenically related to heat-shock protein 72

Andrea Delpino, Anna Maria Mileo, Vincenza Lapenta, Pierluca Piselli, Alessandra Verdina, Lorenzo Polenzani

Research output: Contribution to journalArticle

Abstract

M-14 human melanoma cells, following severe hyperthermic exposures, synthesized a heat-shock protein of 66 kDa (hsp 66), in addition to the major 'classic' heat-shock proteins. This hsp 66 was not expressed following mild hyperthermic exposures sufficient to trigger the synthesis of the other heat- shock proteins. The induction of hsp 66 was observed also in Li human glioma cells treated at 45°C for 20 min. By contrast, hsp 66 was not induced in seven other human cell lines (both melanoma and nonmelanoma) when they were subjected to the same hyperthermic treatment. Immunological recognition experiments showed that hsp 66 cross-reacted with the inducible hsp 72, but not with the constitutive hsp 73. The possibility that hsp 66 is a breakdown product of hsp 72 was ruled out by the fact that Poly(A)+ RNA extracted from cells treated at 45°C for 20 min was able to direct the synthesis of hsp 66 (together with hsp 72) in a message-dependent rabbit reticulocyte lysate, as well as in microinjected Xenopus oocytes. By contrast, only the hsp 72 was expressed using Poly(A)+ RNA extracted from cells heated at 42°C for 1 h. Affinity chromatography experiments on ATP-agarose showed that hsp 66 did not bind ATP in vitro, hsp 66 was localized both in the cytoplasm (cytosol, mitochondria, and microsome fraction) and in the nuclei of cells recovered from a severe heat shock: this intracellular distribution closely corresponded to that of hsp 72. The nuclear-associated hsp 66 was found to be tightly bound to nuclear structures and could not be extracted by incubation in ATP-containing buffer.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalJournal of Cellular Biochemistry
Volume63
Issue number1
DOIs
Publication statusPublished - Oct 1996

Fingerprint

HSP72 Heat-Shock Proteins
Heat-Shock Proteins
Temperature
Melanoma
Adenosine Triphosphate
Affinity chromatography
Messenger RNA
Mitochondria
Reticulocytes
Xenopus
Microsomes
Cell Nucleus
Affinity Chromatography
Glioma
Cytosol
Oocytes

Keywords

  • heat-shock proteins
  • human cell lines
  • hyperthermia
  • melanoma cells
  • Xenopus

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Characterization of a new high-temperature-induced 66-kDa heat-shock protein, antigenically related to heat-shock protein 72. / Delpino, Andrea; Mileo, Anna Maria; Lapenta, Vincenza; Piselli, Pierluca; Verdina, Alessandra; Polenzani, Lorenzo.

In: Journal of Cellular Biochemistry, Vol. 63, No. 1, 10.1996, p. 51-60.

Research output: Contribution to journalArticle

@article{a11e69918f1c4acd871bcb1e1c07558a,
title = "Characterization of a new high-temperature-induced 66-kDa heat-shock protein, antigenically related to heat-shock protein 72",
abstract = "M-14 human melanoma cells, following severe hyperthermic exposures, synthesized a heat-shock protein of 66 kDa (hsp 66), in addition to the major 'classic' heat-shock proteins. This hsp 66 was not expressed following mild hyperthermic exposures sufficient to trigger the synthesis of the other heat- shock proteins. The induction of hsp 66 was observed also in Li human glioma cells treated at 45°C for 20 min. By contrast, hsp 66 was not induced in seven other human cell lines (both melanoma and nonmelanoma) when they were subjected to the same hyperthermic treatment. Immunological recognition experiments showed that hsp 66 cross-reacted with the inducible hsp 72, but not with the constitutive hsp 73. The possibility that hsp 66 is a breakdown product of hsp 72 was ruled out by the fact that Poly(A)+ RNA extracted from cells treated at 45°C for 20 min was able to direct the synthesis of hsp 66 (together with hsp 72) in a message-dependent rabbit reticulocyte lysate, as well as in microinjected Xenopus oocytes. By contrast, only the hsp 72 was expressed using Poly(A)+ RNA extracted from cells heated at 42°C for 1 h. Affinity chromatography experiments on ATP-agarose showed that hsp 66 did not bind ATP in vitro, hsp 66 was localized both in the cytoplasm (cytosol, mitochondria, and microsome fraction) and in the nuclei of cells recovered from a severe heat shock: this intracellular distribution closely corresponded to that of hsp 72. The nuclear-associated hsp 66 was found to be tightly bound to nuclear structures and could not be extracted by incubation in ATP-containing buffer.",
keywords = "heat-shock proteins, human cell lines, hyperthermia, melanoma cells, Xenopus",
author = "Andrea Delpino and Mileo, {Anna Maria} and Vincenza Lapenta and Pierluca Piselli and Alessandra Verdina and Lorenzo Polenzani",
year = "1996",
month = "10",
doi = "10.1002/(SICI)1097-4644(199610)63:1<51::AID-JCB4>3.0.CO;2-Z",
language = "English",
volume = "63",
pages = "51--60",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Characterization of a new high-temperature-induced 66-kDa heat-shock protein, antigenically related to heat-shock protein 72

AU - Delpino, Andrea

AU - Mileo, Anna Maria

AU - Lapenta, Vincenza

AU - Piselli, Pierluca

AU - Verdina, Alessandra

AU - Polenzani, Lorenzo

PY - 1996/10

Y1 - 1996/10

N2 - M-14 human melanoma cells, following severe hyperthermic exposures, synthesized a heat-shock protein of 66 kDa (hsp 66), in addition to the major 'classic' heat-shock proteins. This hsp 66 was not expressed following mild hyperthermic exposures sufficient to trigger the synthesis of the other heat- shock proteins. The induction of hsp 66 was observed also in Li human glioma cells treated at 45°C for 20 min. By contrast, hsp 66 was not induced in seven other human cell lines (both melanoma and nonmelanoma) when they were subjected to the same hyperthermic treatment. Immunological recognition experiments showed that hsp 66 cross-reacted with the inducible hsp 72, but not with the constitutive hsp 73. The possibility that hsp 66 is a breakdown product of hsp 72 was ruled out by the fact that Poly(A)+ RNA extracted from cells treated at 45°C for 20 min was able to direct the synthesis of hsp 66 (together with hsp 72) in a message-dependent rabbit reticulocyte lysate, as well as in microinjected Xenopus oocytes. By contrast, only the hsp 72 was expressed using Poly(A)+ RNA extracted from cells heated at 42°C for 1 h. Affinity chromatography experiments on ATP-agarose showed that hsp 66 did not bind ATP in vitro, hsp 66 was localized both in the cytoplasm (cytosol, mitochondria, and microsome fraction) and in the nuclei of cells recovered from a severe heat shock: this intracellular distribution closely corresponded to that of hsp 72. The nuclear-associated hsp 66 was found to be tightly bound to nuclear structures and could not be extracted by incubation in ATP-containing buffer.

AB - M-14 human melanoma cells, following severe hyperthermic exposures, synthesized a heat-shock protein of 66 kDa (hsp 66), in addition to the major 'classic' heat-shock proteins. This hsp 66 was not expressed following mild hyperthermic exposures sufficient to trigger the synthesis of the other heat- shock proteins. The induction of hsp 66 was observed also in Li human glioma cells treated at 45°C for 20 min. By contrast, hsp 66 was not induced in seven other human cell lines (both melanoma and nonmelanoma) when they were subjected to the same hyperthermic treatment. Immunological recognition experiments showed that hsp 66 cross-reacted with the inducible hsp 72, but not with the constitutive hsp 73. The possibility that hsp 66 is a breakdown product of hsp 72 was ruled out by the fact that Poly(A)+ RNA extracted from cells treated at 45°C for 20 min was able to direct the synthesis of hsp 66 (together with hsp 72) in a message-dependent rabbit reticulocyte lysate, as well as in microinjected Xenopus oocytes. By contrast, only the hsp 72 was expressed using Poly(A)+ RNA extracted from cells heated at 42°C for 1 h. Affinity chromatography experiments on ATP-agarose showed that hsp 66 did not bind ATP in vitro, hsp 66 was localized both in the cytoplasm (cytosol, mitochondria, and microsome fraction) and in the nuclei of cells recovered from a severe heat shock: this intracellular distribution closely corresponded to that of hsp 72. The nuclear-associated hsp 66 was found to be tightly bound to nuclear structures and could not be extracted by incubation in ATP-containing buffer.

KW - heat-shock proteins

KW - human cell lines

KW - hyperthermia

KW - melanoma cells

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0029739363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029739363&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4644(199610)63:1<51::AID-JCB4>3.0.CO;2-Z

DO - 10.1002/(SICI)1097-4644(199610)63:1<51::AID-JCB4>3.0.CO;2-Z

M3 - Article

C2 - 8891903

AN - SCOPUS:0029739363

VL - 63

SP - 51

EP - 60

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 1

ER -