Characterization of a new HLA-DRB3 allele, DRB3*0217, by direct sequencing

S. Frison; E. Longhi; F. Poli; A. Espadas De Arias; M. Scalamogna

doi:10.1034/j.1399-0039.2002.600211.x

Characterization of a new HLA-DRB3 allele, DRB3*0217, by direct sequencing

S. Frison, E. Longhi, F. Poli, A. Espadas De Arias, M. Scalamogna

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

We report the identification of a novel DRB3*02 using sequence-based typing (SBT). This new allele, officially named DRB3*0217, was detected while performing HLA high resolution typing of a bone marrow recipient and his siblings. DNA sequencing demonstrated the presence of a nucleotide substitution in exon 2 at position 199 where a C was substituted by a T. This point mutation at codon 67 (CTC→TTC) has resulted in an amino acid substitution from Leucine to Phenylalanine.

Original language	English
Pages (from-to)	189-190
Number of pages	2
Journal	Tissue Antigens
Volume	60
Issue number	2
DOIs	https://doi.org/10.1034/j.1399-0039.2002.600211.x
Publication status	Published - Aug 1 2002

Keywords

HLA-DRB3
SBT

ASJC Scopus subject areas

Cell Biology
Immunology

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10.1034/j.1399-0039.2002.600211.x

Cite this

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abstract = "We report the identification of a novel DRB3*02 using sequence-based typing (SBT). This new allele, officially named DRB3*0217, was detected while performing HLA high resolution typing of a bone marrow recipient and his siblings. DNA sequencing demonstrated the presence of a nucleotide substitution in exon 2 at position 199 where a C was substituted by a T. This point mutation at codon 67 (CTC→TTC) has resulted in an amino acid substitution from Leucine to Phenylalanine.",

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AU - Longhi, E.

AU - Poli, F.

AU - Espadas De Arias, A.

AU - Scalamogna, M.

PY - 2002/8/1

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N2 - We report the identification of a novel DRB3*02 using sequence-based typing (SBT). This new allele, officially named DRB3*0217, was detected while performing HLA high resolution typing of a bone marrow recipient and his siblings. DNA sequencing demonstrated the presence of a nucleotide substitution in exon 2 at position 199 where a C was substituted by a T. This point mutation at codon 67 (CTC→TTC) has resulted in an amino acid substitution from Leucine to Phenylalanine.

AB - We report the identification of a novel DRB3*02 using sequence-based typing (SBT). This new allele, officially named DRB3*0217, was detected while performing HLA high resolution typing of a bone marrow recipient and his siblings. DNA sequencing demonstrated the presence of a nucleotide substitution in exon 2 at position 199 where a C was substituted by a T. This point mutation at codon 67 (CTC→TTC) has resulted in an amino acid substitution from Leucine to Phenylalanine.

KW - HLA-DRB3

KW - SBT

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Characterization of a new HLA-DRB3 allele, DRB3*0217, by direct sequencing

Abstract

Keywords

ASJC Scopus subject areas

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