Characterization of a nonspecific activator protein for the enzyme hydrolysis of glycolipids

S. C. Li, S. Sonnino, G. Tettamanti, Y. T. Li

Research output: Contribution to journalArticlepeer-review


We have studied the substrate specificities of a non-specific activator protein on the enzymatic hydrolyses of the following compounds: G(M1) and G(M2), as well as several of their derivatives including oligosaccharides, GgOse3Cer-II3-sulfate and LacCer-II3-sulfate, Gb-Ose3Cer and GgOse4Cer, three neolacto-series glycosphingolipids, and two non-ceramide glycolipids. Our results show that this activator protein has a broad spectrum of activity and exhibits the properties of a nonspecific natural detergent. The evidence of non-specificity was the ability of this activator protein to stimulate the hydrolyses of glycolipids, regardless of glycosphingolipids or non-ceramide glycolipids, carried out by glycosidases from animals, plants, and microorganisms. Its activity was, however, limited to substrates that had a lipid moiety. The oligosaccharide of G(M1) and deacetyl-fatty acid free G(M1) (II3-NeuGg-Ose4-sphingosine) were hydrolyzed by β-galactosidase in the absence of this activator protein.

Original languageEnglish
Pages (from-to)6588-6591
Number of pages4
JournalJournal of Biological Chemistry
Issue number14
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Biochemistry


Dive into the research topics of 'Characterization of a nonspecific activator protein for the enzyme hydrolysis of glycolipids'. Together they form a unique fingerprint.

Cite this