Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas

Donatella Buonfiglio, Manuela Bragardo, Sara Bonissoni, Valter Redoglia, Roberto Cauda, Simona Zupo, Vito L. Burgio, Henrik Wolff, Kaarle Franssila, Gianluca Gaidano, Antonino Carbone, Charles A. Janeway, Umberto Dianzani

Research output: Contribution to journalArticlepeer-review

Abstract

We have previously characterized mouse H4 (mH4), a surface glycoprotein recognized by the C398.4A monoclonal antibody. We now show that C398.4A also binds its human putative homolog (hpH4). Both hpH4 and mH4 (1) are selectively expressed by activated T cells and mature thymocytes, (2) are disulfide-linked dimers of two chains (29/37 kDa in humans, 25/29 kDa in mice), whose N-deglycosylation produces a single band at 20-21 kDa, and (3) display a low association with CD4 and the TCR. The expression pattern of hpH4 and its biochemical features showed that it is different from other known activation molecules, and this was confirmed when analysis of the tryptic digest of the hpH4 29-kDa band by peptide mass searching using matrix-assisted laser desorption ionization mass spectrometry did not reveal any significant homology with other molecules. In normal lymphoid tissue, hpH4 is expressed by T cells located at the periphery of lymph node germinal centers and paracortical areas. In T cell neoplasia, expression of hpH4 clusters with a subset of peripheral T cell lymphomas with a large-cell component, and with cases of angioimmunoblastic T cell lymphomas. Overall, these data provide evidence for a novel T cell activation molecule that could help in the phenotypic categorization of T cell malignancies.

Original languageEnglish
Pages (from-to)2863-2874
Number of pages12
JournalEuropean Journal of Immunology
Volume29
Issue number9
Publication statusPublished - 1999

Keywords

  • Lymphoma
  • T cell activation
  • Thymus

ASJC Scopus subject areas

  • Immunology

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