Characterization of a second promoter for the mouse liver/bone/kidney-type alkaline phosphatase gene: Cell and tissue specific expression

Michela Studer, Mineko Terao, Maurizio Giannì, Enrico Garattini

Research output: Contribution to journalArticlepeer-review

Abstract

The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines of different origin and in various tissues by polymerase chain reaction and RNase mapping analysis. The first promoter is active in embryo derived cells, whereas the second promoter is silent in basal conditions but it is activated by dibutyryl cAMP in fibroblastic cells. In the whole animal, the transcript driven by the first promoter is found in most tissues albeit at different levels, while the one driven by the second promoter is specifically expressed at high levels only in the heart.

Original languageEnglish
Pages (from-to)1352-1360
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number3
DOIs
Publication statusPublished - Sep 30 1991

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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