TY - JOUR
T1 - Characterization of a second promoter for the mouse liver/bone/kidney-type alkaline phosphatase gene
T2 - Cell and tissue specific expression
AU - Studer, Michela
AU - Terao, Mineko
AU - Giannì, Maurizio
AU - Garattini, Enrico
PY - 1991/9/30
Y1 - 1991/9/30
N2 - The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines of different origin and in various tissues by polymerase chain reaction and RNase mapping analysis. The first promoter is active in embryo derived cells, whereas the second promoter is silent in basal conditions but it is activated by dibutyryl cAMP in fibroblastic cells. In the whole animal, the transcript driven by the first promoter is found in most tissues albeit at different levels, while the one driven by the second promoter is specifically expressed at high levels only in the heart.
AB - The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines of different origin and in various tissues by polymerase chain reaction and RNase mapping analysis. The first promoter is active in embryo derived cells, whereas the second promoter is silent in basal conditions but it is activated by dibutyryl cAMP in fibroblastic cells. In the whole animal, the transcript driven by the first promoter is found in most tissues albeit at different levels, while the one driven by the second promoter is specifically expressed at high levels only in the heart.
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U2 - 10.1016/0006-291X(91)91722-O
DO - 10.1016/0006-291X(91)91722-O
M3 - Article
C2 - 1930180
AN - SCOPUS:0026002736
VL - 179
SP - 1352
EP - 1360
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -