Characterization of a Staphylococcus aureus strain showing high levels of biofilm formation isolated from a vascular graft: Case report

D. Petrelli, A. Repetto, M. C. di Luca, B. Parente, V. Tavolini, P. Cao, C. Ripa, M. Prenna, Luca Agostino Vitali

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Abstract

A methicillin-susceptible Staphylococcus aureus strain, SA-DZ1, was isolated from an infected bypass crossover graft. Its general microbiological features were reminiscent of those previously described for the wound Wiley strain. Removal of the prosthetic device was necessary to resolve the infection. SA-DZ1 grown under different conditions showed a very strong and distinctive biofilm-producing phenotype, which was also visualized by confocal laser scanning microscopy. The biofilm extracellular matrix was essentially polysaccharidic, as determined by differential growth and physicochemical tests. By Multi Locus Sequence Typing (MLST), SA-DZ1 was classified as st94, a single locus variant of st8. Several other genetic traits assayed by PCR, such as agr-type and the presence of gene encoding proteins involved in adhesion and virulence (e.g. ica operon), confirmed the identifying features of this clinical isolate.

Original languageEnglish
Pages (from-to)745-750
Number of pages6
JournalInternational Journal of Immunopathology and Pharmacology
Volume21
Issue number3
Publication statusPublished - Jul 2008

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Keywords

  • Biofilm
  • Staphylococcus aureus
  • Vascular graft

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

Petrelli, D., Repetto, A., di Luca, M. C., Parente, B., Tavolini, V., Cao, P., Ripa, C., Prenna, M., & Vitali, L. A. (2008). Characterization of a Staphylococcus aureus strain showing high levels of biofilm formation isolated from a vascular graft: Case report. International Journal of Immunopathology and Pharmacology, 21(3), 745-750.