TY - JOUR
T1 - Characterization of amyloid-β Deposits in Bovine Brains
AU - Costassa, E. Vallino
AU - Fiorini, M.
AU - Zanusso, G.
AU - Peletto, S.
AU - Acutis, Pier Luigi
AU - Baioni, Elisa
AU - Maurella, Cristiana
AU - Tagliavini, Fabrizio
AU - Catania, Marcella
AU - Gallo, Marina
AU - Lo Faro, Monica
AU - Chieppa, M. N.
AU - Meloni, Daniela
AU - D'Angelo, Antonio
AU - Paciello, Orlando
AU - Ghidoni, Roberta
AU - Tonoli, Elisa
AU - Casalone, C.
AU - Corona, Cristiano
PY - 2016/3/30
Y1 - 2016/3/30
N2 - amyloid-β (Aβ) deposits are seen in aged individuals of many mammalian species that possess the same aminoacid sequence as humans. This study describes Aβ deposition in 102 clinically characterized cattle brains from animals aged 0 to 20 years. Extracellular and intracellular Aβ deposition was detected with 4G8 antibody in the cortex, hippocampus, and cerebellum. X-34 staining failed to stain Aβ deposits, indicating the non β-pleated nature of these deposits. Western blot analysis and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry revealed in Tris, Triton, and formic acid fractions the presence of different Aβ peptides, characterized mainly by C-terminally truncated forms. Exploration of the genetic variability of APOE, PSEN1, and PSEN2 genes involved in Alzheimer's disease pathogenesis revealed several previously unreported polymorphisms. This study demonstrates certain similarities between Aβ deposition patterns exhibited in cattle brains and those in the human brain in early stages of aging. Furthermore, the identification of the same Aβ peptides reported in humans, but unable to form aggregates, supports the hypothesis that cattle may be protected against amyloid plaque formation.
AB - amyloid-β (Aβ) deposits are seen in aged individuals of many mammalian species that possess the same aminoacid sequence as humans. This study describes Aβ deposition in 102 clinically characterized cattle brains from animals aged 0 to 20 years. Extracellular and intracellular Aβ deposition was detected with 4G8 antibody in the cortex, hippocampus, and cerebellum. X-34 staining failed to stain Aβ deposits, indicating the non β-pleated nature of these deposits. Western blot analysis and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry revealed in Tris, Triton, and formic acid fractions the presence of different Aβ peptides, characterized mainly by C-terminally truncated forms. Exploration of the genetic variability of APOE, PSEN1, and PSEN2 genes involved in Alzheimer's disease pathogenesis revealed several previously unreported polymorphisms. This study demonstrates certain similarities between Aβ deposition patterns exhibited in cattle brains and those in the human brain in early stages of aging. Furthermore, the identification of the same Aβ peptides reported in humans, but unable to form aggregates, supports the hypothesis that cattle may be protected against amyloid plaque formation.
KW - Aging
KW - amyloid beta-protein
KW - cattle
KW - glial cells
UR - http://www.scopus.com/inward/record.url?scp=84963770409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963770409&partnerID=8YFLogxK
U2 - 10.3233/JAD-151007
DO - 10.3233/JAD-151007
M3 - Article
VL - 51
SP - 875
EP - 887
JO - Journal of Alzheimer's Disease
JF - Journal of Alzheimer's Disease
SN - 1387-2877
IS - 3
ER -