Characterization of an in vitro model to study the possible role of polyomavirus BK in prostate cancer

Sonia Villani, Nicoletta Gagliano, Patrizia Procacci, Patrizia Sartori, Manola Comar, Maurizio Provenzano, Evaldo Favi, Mariano Ferraresso, Pasquale Ferrante, Serena Delbue

Research output: Contribution to journalArticle

Abstract

Prostate cancer (PCa) is the most common male neoplasms in the Western world. Various risk factors may lead to carcinogenesis, including infectious agents such as polyomavirus BK (BKPyV), which infects the human renourinary tract, establishes latency, and encodes oncoproteins. Previous studies suggested that BKPyV plays a role in PCa pathogenesis. However, the unspecific tropism of BKPyV and the lack of in vitro models of BKPyV-infected prostate cells cast doubt on this hypothesis. The aim of the present study was to determine whether BKPyV could (a) infect normal and/or tumoral epithelial prostate cells and (b) affect their phenotype. Normal epithelial prostate RWPE-1 cells and PCa PC-3 cells were infected with BKPyV for 21 days. Cell proliferation, cytokine production, adhesion, invasion ability, and epithelial-to-mesenchymal transition (EMT) markers were analyzed. Our results show that (a) RWPE-1 and PC-3 cells are both infectable with BKPyV, but the outcome of the infection varies, (b) cell proliferation and TNF-α production were increased in BKPyV-infected RWPE-1, but not in PC-3 cells, (c) adhesion to matrigel and invasion abilities were elevated in BKPyV-infected RWPE-1 cells, and (d) loss of E-cadherin and expression of vimentin occurred in both uninfected and infected RWPE-1 cells. In conclusion, BKPyV may change some features of the normal prostate cells but is not needed for maintaining the transformed phenotype in the PCa cells The fact that RWPE-1 cells exhibit some phenotype modifications related to EMT represents a limit of this in vitro model.

Original languageEnglish
Pages (from-to)11912-11922
JournalJournal of Cellular Physiology
Volume234
Issue number7
DOIs
Publication statusPublished - 2019

Fingerprint

BK Virus
Cell proliferation
Prostatic Neoplasms
Adhesion
Oncogene Proteins
Vimentin
Cadherins
Prostate
Cytokines
Epithelial-Mesenchymal Transition
Phenotype
Cell Proliferation
In Vitro Techniques
Western World
Tropism
Cell Adhesion
Carcinogenesis
Epithelial Cells
matrigel

Keywords

  • cytokine production
  • in vitro model
  • polyomavirus
  • prostate Cancer (PCa)

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Villani, S., Gagliano, N., Procacci, P., Sartori, P., Comar, M., Provenzano, M., ... Delbue, S. (2019). Characterization of an in vitro model to study the possible role of polyomavirus BK in prostate cancer. Journal of Cellular Physiology, 234(7), 11912-11922. https://doi.org/10.1002/jcp.27871

Characterization of an in vitro model to study the possible role of polyomavirus BK in prostate cancer. / Villani, Sonia; Gagliano, Nicoletta; Procacci, Patrizia; Sartori, Patrizia; Comar, Manola; Provenzano, Maurizio; Favi, Evaldo; Ferraresso, Mariano; Ferrante, Pasquale; Delbue, Serena.

In: Journal of Cellular Physiology, Vol. 234, No. 7, 2019, p. 11912-11922.

Research output: Contribution to journalArticle

Villani, S, Gagliano, N, Procacci, P, Sartori, P, Comar, M, Provenzano, M, Favi, E, Ferraresso, M, Ferrante, P & Delbue, S 2019, 'Characterization of an in vitro model to study the possible role of polyomavirus BK in prostate cancer', Journal of Cellular Physiology, vol. 234, no. 7, pp. 11912-11922. https://doi.org/10.1002/jcp.27871
Villani, Sonia ; Gagliano, Nicoletta ; Procacci, Patrizia ; Sartori, Patrizia ; Comar, Manola ; Provenzano, Maurizio ; Favi, Evaldo ; Ferraresso, Mariano ; Ferrante, Pasquale ; Delbue, Serena. / Characterization of an in vitro model to study the possible role of polyomavirus BK in prostate cancer. In: Journal of Cellular Physiology. 2019 ; Vol. 234, No. 7. pp. 11912-11922.
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