Characterization of antibodies directed against the immunoglobulin light κ chain variable chain region (VK) of hepatitis C virus-related type-II mixed cryoglobulinemia and B-cell proliferations

Valli De Re, Maria Paola Simula, Alessandro Pavan, Marica Garziera, Dolores Marin, Riccardo Dolcetti, Salvatore De Vita, Domenico Sansonno, Silvano Geremia, Giuseppe Toffoli

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Autoimmune type-II cryoglobulinemia (II-MC) is sustained by hepatitis C virus (HCV) infection and B-cell (oligo)clones. This is the reason why the disease may be considered an "indolent B-cell lymphoma (NHL)." B clones show a restricted use of immunoglobulin variable genes (BCR), in particular in the use of the variable κ (VK)3-2015 light chain, and show a homology between their BCR functional regions and those of autoimmune rheumatoid factors. We underlined the BCR unique repertoire with frequent rheumatoid factor activity also observed in other autoimmune disorders associated with NHL. The immunoglobulin idiotype is a clonal B-cell marker and an ideal target for immunotherapy. Five monoclonal antibodies were produced in our laboratory toward the VK3-20 of a subject with HCV infection and a II-MC-associated NHL. Epitope determination was performed using the epitope excision approach. Monoclonal antibody reactivity was tested in vitro in ELISA, Western blot, and cytofluorimetry. Data confirmed that a panel of antibodies, reactive against shared idiotypes, can be produced from patients with HCV-associated B-cell lymphoproliferative diseases, thus obviating the need to produce an anti-idiotype antibody for each patient.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages152-160
Number of pages9
Volume1173
DOIs
Publication statusPublished - Sep 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1173
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • HCV
  • Idiotype
  • Lymphoma
  • Monoclonal antibody
  • NHL
  • Single-chain immunoglobulin variable fragment
  • Type II mixed cryoglobulinemia

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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