Characterization of antimalarial SPf66 peptide using MALDI-TOF MS, CD and SEC

Alexis Oliva, Maria Jesús Dorta, Ana Santovea, Valentina Bonetto, Mario Salmona, José B. Faria

Research output: Contribution to journalArticlepeer-review


SPf66 is the first chemically synthesized peptide to elicit a partial protective immune response against malaria. Size-exclusion chromatography (SEC) with multi-angle laser light-scattering (MALLS) detection and hydrogen/deuterium (H/D) exchange monitored by (matrix-assisted laser desorption/ionization) MALDI-TOF (time-of-flight) mass spectrometry (MS) were used to assess the conformation and stability in aqueous solution after storage at different temperatures. Moreover, the feasible conformational changes of this peptide were also measured by circular dichroism (CD)-spectroscopy. The absolute molecular weight of SPf66 monomer and dimer species were 4765 and 8960Da using SEC with MALLS detection, and 4643 and 9490Da by MALDI-TOF MS, the discrepancy being between both methods lower than 5.7%, a value quite close to those found in other proteins. The results from H/D exchange monitored by MALDI-TOF MS and CD-spectroscopy show that the SPf66 monomer lacks ordered structure, whereas the SPf66 dimer species presents segments of secondary structure as a determined by CD measurements.

Original languageEnglish
Pages (from-to)1527-1535
Number of pages9
Issue number9
Publication statusPublished - Sep 2002


  • Circular dichroism
  • Molecular mass
  • Multi-angle laser light-scattering
  • SEC
  • Secondary structure
  • SPf66 peptide

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience


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