TY - JOUR
T1 - Characterization of arachidonic acid metabolic profiles in animal tissues by high-resolution gas chromatography-mass spectrometry
AU - Chiabrando, Chiara
AU - Noseda, Alessandro
AU - Castagnoli, M. Novella
AU - Salmona, Mario
AU - Fanelli, Roberto
PY - 1984/7/6
Y1 - 1984/7/6
N2 - A standardized, highly specific routine method was developed for the quantitative profiling of cyclooxygenase metabolites of arachidonic acid in animal tissues. Whole homogenates were used to assess the potential capacity of tissues to metabolize endogenous arachidonic acid. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the selected ion monitoring mode. The screening of several rat tissues by this method revealed marked tissue-specificity in both the synthesis capacity and prostaglandin profile. The major products detected were: 6-ketoprostaglandin F1α for lung, stomach, muscle and heart; prostaglandin D2 for spleen, brain and liver; prostaglandin F2α for kidney and prostaglandin E2 for seminal vesicles. Marked species differences were found when guinea pig tissues were analyzed.
AB - A standardized, highly specific routine method was developed for the quantitative profiling of cyclooxygenase metabolites of arachidonic acid in animal tissues. Whole homogenates were used to assess the potential capacity of tissues to metabolize endogenous arachidonic acid. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the selected ion monitoring mode. The screening of several rat tissues by this method revealed marked tissue-specificity in both the synthesis capacity and prostaglandin profile. The major products detected were: 6-ketoprostaglandin F1α for lung, stomach, muscle and heart; prostaglandin D2 for spleen, brain and liver; prostaglandin F2α for kidney and prostaglandin E2 for seminal vesicles. Marked species differences were found when guinea pig tissues were analyzed.
KW - Arachidonic acid metabolite
KW - Cyclooxygenase
KW - GC-MS
KW - Prostaglandin
UR - http://www.scopus.com/inward/record.url?scp=0021192042&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021192042&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(84)90158-9
DO - 10.1016/0005-2760(84)90158-9
M3 - Article
C2 - 6428459
AN - SCOPUS:0021192042
VL - 794
SP - 292
EP - 297
JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
SN - 0005-2760
IS - 2
ER -