Characterization of C2C12 cells in simulated microgravity: Possible use for myoblast regeneration

Daniela Calzia, Laura Ottaggio, Alessandro Cora, Giorgia Chiappori, Paola Cuccarolo, Enrico Cappelli, Alberto Izzotti, Sara Tavella, Paolo Degan

Research output: Contribution to journalArticle

Abstract

Muscle loss is a major problem for many in lifetime. Muscle and bone degeneration has also been observed in individuals exposed to microgravity and in unloading conditions. C2C12 myoblst cells are able to form myotubes, and myofibers and these cells have been employed for muscle regeneration purposes and in myogenic regeneration and transplantation studies. We exposed C2C12 cells in an random position machine to simulate microgravity and study the energy and the biochemical challenges associated with this treatment. Simulated microgravity exposed C2C12 cells maintain positive proliferation indices and delay the differentiation process for several days. On the other hand this treatment significantly alters many of the biochemical and the metabolic characteristics of the cell cultures including calcium homeostasis. Recent data have shown that these perturbations are due to the inhibition of the ryanodine receptors on the membranes of intracellular calcium stores. We were able to reverse this perturbations treating cells with thapsigargin which prevents the segregation of intracellular calcium ions in the mitochondria and in the sarco/endoplasmic reticula. Calcium homeostasis appear a key target of microgravity exposure. In conclusion, in this study we reported some of the effects induced by the exposure of C2C12 cell cultures to simulated microgravity. The promising information obtained is of fundamental importance in the hope to employ this protocol in the field of regenerative medicine.

Original languageEnglish
JournalJournal of Cellular Physiology
DOIs
Publication statusAccepted/In press - Jan 1 2019

Fingerprint

Weightlessness
Myoblasts
Microgravity
Regeneration
Calcium
Muscle
Cell culture
Muscles
Homeostasis
Cell Culture Techniques
Intracellular Membranes
Ryanodine Receptor Calcium Release Channel
Mitochondria
Regenerative Medicine
Thapsigargin
Skeletal Muscle Fibers
Unloading
Endoplasmic Reticulum
Bone
Transplantation

Keywords

  • biochemistry
  • C2C12 myoblasts
  • cancer-related lesions
  • cell biology
  • microgravity
  • regenerative medicine

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Characterization of C2C12 cells in simulated microgravity : Possible use for myoblast regeneration. / Calzia, Daniela; Ottaggio, Laura; Cora, Alessandro; Chiappori, Giorgia; Cuccarolo, Paola; Cappelli, Enrico; Izzotti, Alberto; Tavella, Sara; Degan, Paolo.

In: Journal of Cellular Physiology, 01.01.2019.

Research output: Contribution to journalArticle

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