Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells

Giuseppe Gaipa, Elaine Coustan-Smith, Elisabetta Todisco, Oscar Maglia, Andrea Biondi, Dario Campana

Research output: Contribution to journalArticle

Abstract

Background and Objectives. Hematopoietic progenitor cells that express CD34 are heterogeneous in their lineage affiliation and degree of maturation. Expression of CD13 and CD33 antigens indicates myeloid lineage association, but the precise sequence of expression of these two markers during differentiation is unclear. We noted the presence of CD34+ cells expressing CD13 but lacking CD33, a subset of cells not yet well characterized. In this report we describe the prevalence and the immunophenotype of this cell subset. Design and Methods. We studied the immunophenotype of immature myeloid cells in human bone marrow samples from 11 healthy transplantation donors and in 4 cord blood samples. We used four-colorflow cytometry and a large panel of monoclonal antibodies directed against lineage and differentiation-associated antigens. Three additional bone marrow samples were analyzed after immunomagnetic sorting of CD34+ cells. We focused our analysis on the subset of cells defined by the expression of CD34 and CD13 and the lack of CD33. Results. We found CD34+, CD13+, CD33- cells in all 11 bone marrow and 4 cord blood samples studied. These cells represented 0.5±0.5% (mean ± SD) and 0.8±1.2% of mononucleated cells, respectively. CD34+, CD13+, CD33- cells appeared to be more immature than those expressing CD33 because of their light scatter characteristics (smaller size and lower granularity), the expression of markers associated with early hematopoietic cells (CD90, CD133 and CD117), and the absence of lineage-associated markers. Interpretation and Conclusions. These findings suggest that the expression of CD13 precedes that of CD33 during myeloid differentiation, and that CD34+, CD13+, CD33- cells are at an early stage of human myeloid cell differentiation.

Original languageEnglish
Pages (from-to)347-356
Number of pages10
JournalHaematologica
Volume87
Issue number4
Publication statusPublished - 2002

Fingerprint

Bone Marrow
Differentiation Antigens
Myeloid Cells
Fetal Blood
Sialic Acid Binding Ig-like Lectin 3
CD13 Antigens
Hematopoietic Stem Cells
Cell Differentiation
Transplantation
Monoclonal Antibodies
Light

Keywords

  • CD34 cells
  • Flow cytometry
  • Hematopoiesis
  • Leukemic myeloblasts
  • Minimal residual disease

ASJC Scopus subject areas

  • Hematology

Cite this

Gaipa, G., Coustan-Smith, E., Todisco, E., Maglia, O., Biondi, A., & Campana, D. (2002). Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells. Haematologica, 87(4), 347-356.

Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells. / Gaipa, Giuseppe; Coustan-Smith, Elaine; Todisco, Elisabetta; Maglia, Oscar; Biondi, Andrea; Campana, Dario.

In: Haematologica, Vol. 87, No. 4, 2002, p. 347-356.

Research output: Contribution to journalArticle

Gaipa, G, Coustan-Smith, E, Todisco, E, Maglia, O, Biondi, A & Campana, D 2002, 'Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells', Haematologica, vol. 87, no. 4, pp. 347-356.
Gaipa, Giuseppe ; Coustan-Smith, Elaine ; Todisco, Elisabetta ; Maglia, Oscar ; Biondi, Andrea ; Campana, Dario. / Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells. In: Haematologica. 2002 ; Vol. 87, No. 4. pp. 347-356.
@article{8f1ff88633d4403899db66861f5aae63,
title = "Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells",
abstract = "Background and Objectives. Hematopoietic progenitor cells that express CD34 are heterogeneous in their lineage affiliation and degree of maturation. Expression of CD13 and CD33 antigens indicates myeloid lineage association, but the precise sequence of expression of these two markers during differentiation is unclear. We noted the presence of CD34+ cells expressing CD13 but lacking CD33, a subset of cells not yet well characterized. In this report we describe the prevalence and the immunophenotype of this cell subset. Design and Methods. We studied the immunophenotype of immature myeloid cells in human bone marrow samples from 11 healthy transplantation donors and in 4 cord blood samples. We used four-colorflow cytometry and a large panel of monoclonal antibodies directed against lineage and differentiation-associated antigens. Three additional bone marrow samples were analyzed after immunomagnetic sorting of CD34+ cells. We focused our analysis on the subset of cells defined by the expression of CD34 and CD13 and the lack of CD33. Results. We found CD34+, CD13+, CD33- cells in all 11 bone marrow and 4 cord blood samples studied. These cells represented 0.5±0.5{\%} (mean ± SD) and 0.8±1.2{\%} of mononucleated cells, respectively. CD34+, CD13+, CD33- cells appeared to be more immature than those expressing CD33 because of their light scatter characteristics (smaller size and lower granularity), the expression of markers associated with early hematopoietic cells (CD90, CD133 and CD117), and the absence of lineage-associated markers. Interpretation and Conclusions. These findings suggest that the expression of CD13 precedes that of CD33 during myeloid differentiation, and that CD34+, CD13+, CD33- cells are at an early stage of human myeloid cell differentiation.",
keywords = "CD34 cells, Flow cytometry, Hematopoiesis, Leukemic myeloblasts, Minimal residual disease",
author = "Giuseppe Gaipa and Elaine Coustan-Smith and Elisabetta Todisco and Oscar Maglia and Andrea Biondi and Dario Campana",
year = "2002",
language = "English",
volume = "87",
pages = "347--356",
journal = "Haematologica",
issn = "0390-6078",
publisher = "NLM (Medline)",
number = "4",

}

TY - JOUR

T1 - Characterization of CD34+, CD13+, CD33- cells, a rare subset of immature human hematopoietic cells

AU - Gaipa, Giuseppe

AU - Coustan-Smith, Elaine

AU - Todisco, Elisabetta

AU - Maglia, Oscar

AU - Biondi, Andrea

AU - Campana, Dario

PY - 2002

Y1 - 2002

N2 - Background and Objectives. Hematopoietic progenitor cells that express CD34 are heterogeneous in their lineage affiliation and degree of maturation. Expression of CD13 and CD33 antigens indicates myeloid lineage association, but the precise sequence of expression of these two markers during differentiation is unclear. We noted the presence of CD34+ cells expressing CD13 but lacking CD33, a subset of cells not yet well characterized. In this report we describe the prevalence and the immunophenotype of this cell subset. Design and Methods. We studied the immunophenotype of immature myeloid cells in human bone marrow samples from 11 healthy transplantation donors and in 4 cord blood samples. We used four-colorflow cytometry and a large panel of monoclonal antibodies directed against lineage and differentiation-associated antigens. Three additional bone marrow samples were analyzed after immunomagnetic sorting of CD34+ cells. We focused our analysis on the subset of cells defined by the expression of CD34 and CD13 and the lack of CD33. Results. We found CD34+, CD13+, CD33- cells in all 11 bone marrow and 4 cord blood samples studied. These cells represented 0.5±0.5% (mean ± SD) and 0.8±1.2% of mononucleated cells, respectively. CD34+, CD13+, CD33- cells appeared to be more immature than those expressing CD33 because of their light scatter characteristics (smaller size and lower granularity), the expression of markers associated with early hematopoietic cells (CD90, CD133 and CD117), and the absence of lineage-associated markers. Interpretation and Conclusions. These findings suggest that the expression of CD13 precedes that of CD33 during myeloid differentiation, and that CD34+, CD13+, CD33- cells are at an early stage of human myeloid cell differentiation.

AB - Background and Objectives. Hematopoietic progenitor cells that express CD34 are heterogeneous in their lineage affiliation and degree of maturation. Expression of CD13 and CD33 antigens indicates myeloid lineage association, but the precise sequence of expression of these two markers during differentiation is unclear. We noted the presence of CD34+ cells expressing CD13 but lacking CD33, a subset of cells not yet well characterized. In this report we describe the prevalence and the immunophenotype of this cell subset. Design and Methods. We studied the immunophenotype of immature myeloid cells in human bone marrow samples from 11 healthy transplantation donors and in 4 cord blood samples. We used four-colorflow cytometry and a large panel of monoclonal antibodies directed against lineage and differentiation-associated antigens. Three additional bone marrow samples were analyzed after immunomagnetic sorting of CD34+ cells. We focused our analysis on the subset of cells defined by the expression of CD34 and CD13 and the lack of CD33. Results. We found CD34+, CD13+, CD33- cells in all 11 bone marrow and 4 cord blood samples studied. These cells represented 0.5±0.5% (mean ± SD) and 0.8±1.2% of mononucleated cells, respectively. CD34+, CD13+, CD33- cells appeared to be more immature than those expressing CD33 because of their light scatter characteristics (smaller size and lower granularity), the expression of markers associated with early hematopoietic cells (CD90, CD133 and CD117), and the absence of lineage-associated markers. Interpretation and Conclusions. These findings suggest that the expression of CD13 precedes that of CD33 during myeloid differentiation, and that CD34+, CD13+, CD33- cells are at an early stage of human myeloid cell differentiation.

KW - CD34 cells

KW - Flow cytometry

KW - Hematopoiesis

KW - Leukemic myeloblasts

KW - Minimal residual disease

UR - http://www.scopus.com/inward/record.url?scp=0036218047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036218047&partnerID=8YFLogxK

M3 - Article

C2 - 11940478

AN - SCOPUS:0036218047

VL - 87

SP - 347

EP - 356

JO - Haematologica

JF - Haematologica

SN - 0390-6078

IS - 4

ER -