Characterization of copine VII, a new member of the copine family, and its exclusion as a candidate in sporadic breast cancers with loss of heterozygosity at 16q24.3

Maria Savino, Maria D'Apolito, Marta Centra, Hetty M. Van Beerendonk, Anne Marie Cleton-Jansen, Scott A. Whitmore, Joanna Crawford, David F. Callen, Leopoldo Zelante, Anna Savoia

Research output: Contribution to journalArticlepeer-review

Abstract

In a search for candidate tumor suppressor genes within a 650-kb common region of loss of heterozygosity (LOH) at 16q24.3 in breast cancer tissues, a 2.6-kb cDNA, named copine VII (CPNE7), was characterized. The gene is 2654 bp and codes for a 633-residue protein with high homology to the other members of the copine family, such as copine I, copine III, and N-copine. The predicted amino acid sequence contains two copies of a C2 domain in the N- terminus. Since these domains have been found in several membrane-binding proteins involved in different intracellular processes, copine VII was viewed as a potential tumor suppressor gene. Mutation analysis was carried out by single-strand conformation polymorphism analysis of 18 breast tumor tissue samples with ascertained LOH on chromosome 16q24.3. Since only two polymorphisms were identified, no evidence was found to indicate that copine VII is the tumor suppressor gene at 16q24.3 involved in breast cancer.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalGenomics
Volume61
Issue number2
DOIs
Publication statusPublished - Oct 15 1999

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Characterization of copine VII, a new member of the copine family, and its exclusion as a candidate in sporadic breast cancers with loss of heterozygosity at 16q24.3'. Together they form a unique fingerprint.

Cite this