Characterization of EBV-positive lymphoblastoid cell lines obtained from HIV seropositive patients with or without lymphomas

Silvio Roncella, Paola Francia Di Celle, Giovanna Cutrona, Anna Carbone, Mario Sessarego, Ferdinando Dodi, Robin Foa, Martin Rowe, Manlio Ferrarini

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Abstract

Epstein-Barr-virus- (EBV-) positive lymphoblastoid cell lines (LCLs) spontaneously arising in vitro were obtained from the peripheral blood of six HIV-seropositive patients and from the peripheral blood and the bone marrow of one patient (LAM) with AIDS and lymphoma. The LCLs from HIV-seropositive patients had phenotypic, cytogenetic, and biological characteristics indistin-guishable from those of normal LCLs obtained by infecting B cells with EBV in vitro. The LCLs from LAM patient comprised composite cell populations. Cloning analysis and cell fractionation procedures showed that, beside normal EBV-infected cells, these lines contained a malignant subset population characterized by c-myc rearrangement, abnormal karyotype, and a surface phenotype similar to that of Burkitt's lymphoma cells. Analyses of Ig heavy chain and c-myc oncogene loci showed that these malignant cells were the progeny of a single precursor. Nevertheless, these cells had heterogeneous EBV-fused termini, a finding which indicates that EBV infection followed c-myc rearrangement.

Original languageEnglish
JournalLeukemia
Volume6
Issue numberSUPPL. 3
Publication statusPublished - 1992

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ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Roncella, S., Francia Di Celle, P., Cutrona, G., Carbone, A., Sessarego, M., Dodi, F., Foa, R., Rowe, M., & Ferrarini, M. (1992). Characterization of EBV-positive lymphoblastoid cell lines obtained from HIV seropositive patients with or without lymphomas. Leukemia, 6(SUPPL. 3).