Abstract
Epstein-Barr-virus- (EBV-) positive lymphoblastoid cell lines (LCLs) spontaneously arising in vitro were obtained from the peripheral blood of six HIV-seropositive patients and from the peripheral blood and the bone marrow of one patient (LAM) with AIDS and lymphoma. The LCLs from HIV-seropositive patients had phenotypic, cytogenetic, and biological characteristics indistin-guishable from those of normal LCLs obtained by infecting B cells with EBV in vitro. The LCLs from LAM patient comprised composite cell populations. Cloning analysis and cell fractionation procedures showed that, beside normal EBV-infected cells, these lines contained a malignant subset population characterized by c-myc rearrangement, abnormal karyotype, and a surface phenotype similar to that of Burkitt's lymphoma cells. Analyses of Ig heavy chain and c-myc oncogene loci showed that these malignant cells were the progeny of a single precursor. Nevertheless, these cells had heterogeneous EBV-fused termini, a finding which indicates that EBV infection followed c-myc rearrangement.
| Original language | English |
|---|---|
| Journal | Leukemia |
| Volume | 6 |
| Issue number | SUPPL. 3 |
| Publication status | Published - 1992 |
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ASJC Scopus subject areas
- Hematology
- Cancer Research
Cite this
Characterization of EBV-positive lymphoblastoid cell lines obtained from HIV seropositive patients with or without lymphomas. / Roncella, Silvio; Francia Di Celle, Paola; Cutrona, Giovanna; Carbone, Anna; Sessarego, Mario; Dodi, Ferdinando; Foa, Robin; Rowe, Martin; Ferrarini, Manlio.
In: Leukemia, Vol. 6, No. SUPPL. 3, 1992.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Characterization of EBV-positive lymphoblastoid cell lines obtained from HIV seropositive patients with or without lymphomas
AU - Roncella, Silvio
AU - Francia Di Celle, Paola
AU - Cutrona, Giovanna
AU - Carbone, Anna
AU - Sessarego, Mario
AU - Dodi, Ferdinando
AU - Foa, Robin
AU - Rowe, Martin
AU - Ferrarini, Manlio
PY - 1992
Y1 - 1992
N2 - Epstein-Barr-virus- (EBV-) positive lymphoblastoid cell lines (LCLs) spontaneously arising in vitro were obtained from the peripheral blood of six HIV-seropositive patients and from the peripheral blood and the bone marrow of one patient (LAM) with AIDS and lymphoma. The LCLs from HIV-seropositive patients had phenotypic, cytogenetic, and biological characteristics indistin-guishable from those of normal LCLs obtained by infecting B cells with EBV in vitro. The LCLs from LAM patient comprised composite cell populations. Cloning analysis and cell fractionation procedures showed that, beside normal EBV-infected cells, these lines contained a malignant subset population characterized by c-myc rearrangement, abnormal karyotype, and a surface phenotype similar to that of Burkitt's lymphoma cells. Analyses of Ig heavy chain and c-myc oncogene loci showed that these malignant cells were the progeny of a single precursor. Nevertheless, these cells had heterogeneous EBV-fused termini, a finding which indicates that EBV infection followed c-myc rearrangement.
AB - Epstein-Barr-virus- (EBV-) positive lymphoblastoid cell lines (LCLs) spontaneously arising in vitro were obtained from the peripheral blood of six HIV-seropositive patients and from the peripheral blood and the bone marrow of one patient (LAM) with AIDS and lymphoma. The LCLs from HIV-seropositive patients had phenotypic, cytogenetic, and biological characteristics indistin-guishable from those of normal LCLs obtained by infecting B cells with EBV in vitro. The LCLs from LAM patient comprised composite cell populations. Cloning analysis and cell fractionation procedures showed that, beside normal EBV-infected cells, these lines contained a malignant subset population characterized by c-myc rearrangement, abnormal karyotype, and a surface phenotype similar to that of Burkitt's lymphoma cells. Analyses of Ig heavy chain and c-myc oncogene loci showed that these malignant cells were the progeny of a single precursor. Nevertheless, these cells had heterogeneous EBV-fused termini, a finding which indicates that EBV infection followed c-myc rearrangement.
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M3 - Article
VL - 6
JO - Leukemia
JF - Leukemia
SN - 0887-6924
IS - SUPPL. 3
ER -