Abstract
Monoclonal antibodies (mAbs) have been used successfully both in research and for clinical purposes. The possible use of protective mAbs directed against different microbial pathogens is currently being considered. The fine definition of the epitope recognized by a protective mAb is an important aspect to be considered for possible development in epitope-based vaccinology. The most accurate approach to this is the X-ray resolution of mAb/antigen crystal complex. Unfortunately, this approach is not always feasible. Under this perspective, several surrogate epitope mapping strategies based on the use of bioinformatics have been developed. In this article, we review the most common, freely accessible, bioinformatic tools used for epitope characterization and provide some basic examples of molecular visualization, editing and computational analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 464-471 |
| Number of pages | 8 |
| Journal | Drug Discovery Today |
| Volume | 18 |
| Issue number | 9-10 |
| DOIs | |
| Publication status | Published - May 2013 |
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ASJC Scopus subject areas
- Drug Discovery
- Pharmacology
Cite this
Characterization of epitopes recognized by monoclonal antibodies : Experimental approaches supported by freely accessible bioinformatic tools. / Clementi, Nicola; Mancini, Nicasio; Castelli, Matteo; Clementi, Massimo; Burioni, Roberto.
In: Drug Discovery Today, Vol. 18, No. 9-10, 05.2013, p. 464-471.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of epitopes recognized by monoclonal antibodies
T2 - Experimental approaches supported by freely accessible bioinformatic tools
AU - Clementi, Nicola
AU - Mancini, Nicasio
AU - Castelli, Matteo
AU - Clementi, Massimo
AU - Burioni, Roberto
PY - 2013/5
Y1 - 2013/5
N2 - Monoclonal antibodies (mAbs) have been used successfully both in research and for clinical purposes. The possible use of protective mAbs directed against different microbial pathogens is currently being considered. The fine definition of the epitope recognized by a protective mAb is an important aspect to be considered for possible development in epitope-based vaccinology. The most accurate approach to this is the X-ray resolution of mAb/antigen crystal complex. Unfortunately, this approach is not always feasible. Under this perspective, several surrogate epitope mapping strategies based on the use of bioinformatics have been developed. In this article, we review the most common, freely accessible, bioinformatic tools used for epitope characterization and provide some basic examples of molecular visualization, editing and computational analysis.
AB - Monoclonal antibodies (mAbs) have been used successfully both in research and for clinical purposes. The possible use of protective mAbs directed against different microbial pathogens is currently being considered. The fine definition of the epitope recognized by a protective mAb is an important aspect to be considered for possible development in epitope-based vaccinology. The most accurate approach to this is the X-ray resolution of mAb/antigen crystal complex. Unfortunately, this approach is not always feasible. Under this perspective, several surrogate epitope mapping strategies based on the use of bioinformatics have been developed. In this article, we review the most common, freely accessible, bioinformatic tools used for epitope characterization and provide some basic examples of molecular visualization, editing and computational analysis.
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U2 - 10.1016/j.drudis.2012.11.006
DO - 10.1016/j.drudis.2012.11.006
M3 - Article
VL - 18
SP - 464
EP - 471
JO - Drug Discovery Today
JF - Drug Discovery Today
SN - 1359-6446
IS - 9-10
ER -