Characterization of growth parameters of a human hepatoma cell line cultured under serum-free conditions

Patrizia Bagnarelli, Marina Brugia, Aldo Manzin, Massimo Clementi

Research output: Contribution to journalArticlepeer-review

Abstract

The PLC/PRF/5 human hepatoma cell line has at least four complete series of hepatitis B virus (HBV)-DNA sequences integrated into the host DNA and produces hepatitis B surface antigen (HBsAg) and several serum proteins in the culture medium. In order to study serum proteins and HBsAg released by these cells under controlled conditions, the serum-free growth of several human hepatoma-derived cell lines was recently investigated. In this paper the growth of PLC/PRF/5 human hepatoma cell line in a serum- and hormone-free medium was investigated. The results represent a tool which might be used in pharmacological research, studies on hormone-binding and virus gene expression.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalLa Ricerca in Clinica e in Laboratorio
Volume15
Issue number2
DOIs
Publication statusPublished - Apr 1985

Keywords

  • HBV-DNA
  • Hepatitis B surface antigen
  • Hepatitis B virus
  • Hepatoma cell lines
  • Hormones
  • Serum proteins

ASJC Scopus subject areas

  • Clinical Biochemistry

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