Characterization of human and mouse c-fes cDNA clones and identification of the 5′ end of the gene

Myriam Alcalay, Francesco Antolini, Wim J. Van De Ven, Luisa Lanfrancone, Fausto Grignani, Pier Giuseppe Pelicci

Research output: Contribution to journalArticle

Abstract

cDNA clones of the human c-fes mRNA were isolated. Nucleotide analysis showed that c-fes mRNA contains a 2514 nucleotide open reading frame, which could encode for a 93kDa protein, and both 5′ and 3′ v-fes non-homologous sequences. Primer extension experiments confirmed that the longest isolated cDNAs are about the same length as the entire human c-fes mRNA. Sequence comparison between human c-fes cDNA and the corresponding genomic regions identified a 5′ viral-non homologous exon (exon 1) located 491 bp upstream from the first v-fes homologous exon. The genomic region surrounding c-fes exon 1 contains a CpG island and acts as a promoter in vitro. Analysis of the 5′ end of mouse c-fes cDNA suggested that the 5′ human and mouse gene structure are similar.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalOncogene
Volume5
Issue number3
Publication statusPublished - Mar 1990

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ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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