Characterization of IgD. I. Isolation of two molecular forms from human serum

G. Corte, P. Tonda, E. Cosulich, C. P. Milstein, A. Bargellesi, M. Ferrarini

Research output: Contribution to journalArticle

Abstract

Human IgD present in the serum of normal individuals or of patients with Hodgkin's disease (having high IgD concentrations) was characterized and compared with five IgD myeloma proteins. IgD was isolated using a highly specific anti-δ insoluble immunoabsorbent from which the bound material was eluted with sodium dodecyl sulphate (SDS) or urea. The latter reagent could be removed by extensive dialysis, thus making possible the renaturation of the eluted molecules. The purity of the IgD thus isolated was confirmed by antigenic analysis. Both κ and λ light chain determinants were present on serum IgD, although λ light chain was predominant with a ratio of the κ chain of 2:1. SDS-polyacrylamide slab gel electrophoresis analysis revealed two different molecular forms of serum IgD, one (IgD1) migrating identically to monoclonal IgD, the other (IgD2) having a faster mobility. The difference between the two molecules was entirely, due to the different sizes of their constituent δ chains. Peptide mapping of the two chains (δ1 and δ2 respectively) and of the δ chain of an IgD myeloma protein was carried out using 125I-labelled material. The three molecules displayed a high degree of homology, the δ2 chain differing by the presence of three characteristic extra peptides. The significance of these extra peptides is discussed in the light of the peptide mapping technique employed.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalScandinavian Journal of Immunology
Volume9
Issue number2
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Immunology

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    Corte, G., Tonda, P., Cosulich, E., Milstein, C. P., Bargellesi, A., & Ferrarini, M. (1979). Characterization of IgD. I. Isolation of two molecular forms from human serum. Scandinavian Journal of Immunology, 9(2), 141-149.