Abstract
This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the J2 (v-raf/v-myc) recombinant murine retrovirus. ANA-1 macrophages did not constitutively express detectable levels of mRNA for the p55, IL-2Rα. However, a brief exposure to IFN-γ was sufficient to induce IL-2Rα mRNA in ANA-1 macrophages. Flow cytometric analysis indicated that ANA-1 macrophages expressed low constitutive levels of IL-2Rα on their cell surface that were augmented after treatment of the cells with IFN-γ. Affinity binding and cross-linking of [125I]IL-2 to ANA-1 macrophages demonstrated that IL-2Rα and the p70-75, IL-2Rβ were both present on ANA-1 macrophages constitutively. IFN-γ increased the expression of IL-2Rα on ANA-1 macrophages but did not increase the expression of IL-2Rβ on these macrophages. Although IL-2 alone did not induce the tumoricidal activity of ANA-1 macrophages, IL-2 acted synergistically with IFN-γ to induce macrophage tumoricidal activity. These data demonstrate the expression of IL-2R on murine macrophage cell lines and establish the role of IL-2 as a costimulator of macrophage-mediated tumoricidal activity.
| Original language | English |
|---|---|
| Pages (from-to) | 1719-1726 |
| Number of pages | 8 |
| Journal | Journal of Immunology |
| Volume | 145 |
| Issue number | 6 |
| Publication status | Published - 1990 |
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ASJC Scopus subject areas
- Immunology
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Characterization of IL-2 receptor expression and function on murine macrophages. / Cox, G. W.; Mathieson, B. J.; Giardina, S. L.; Varesio, L.
In: Journal of Immunology, Vol. 145, No. 6, 1990, p. 1719-1726.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of IL-2 receptor expression and function on murine macrophages
AU - Cox, G. W.
AU - Mathieson, B. J.
AU - Giardina, S. L.
AU - Varesio, L.
PY - 1990
Y1 - 1990
N2 - This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the J2 (v-raf/v-myc) recombinant murine retrovirus. ANA-1 macrophages did not constitutively express detectable levels of mRNA for the p55, IL-2Rα. However, a brief exposure to IFN-γ was sufficient to induce IL-2Rα mRNA in ANA-1 macrophages. Flow cytometric analysis indicated that ANA-1 macrophages expressed low constitutive levels of IL-2Rα on their cell surface that were augmented after treatment of the cells with IFN-γ. Affinity binding and cross-linking of [125I]IL-2 to ANA-1 macrophages demonstrated that IL-2Rα and the p70-75, IL-2Rβ were both present on ANA-1 macrophages constitutively. IFN-γ increased the expression of IL-2Rα on ANA-1 macrophages but did not increase the expression of IL-2Rβ on these macrophages. Although IL-2 alone did not induce the tumoricidal activity of ANA-1 macrophages, IL-2 acted synergistically with IFN-γ to induce macrophage tumoricidal activity. These data demonstrate the expression of IL-2R on murine macrophage cell lines and establish the role of IL-2 as a costimulator of macrophage-mediated tumoricidal activity.
AB - This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the J2 (v-raf/v-myc) recombinant murine retrovirus. ANA-1 macrophages did not constitutively express detectable levels of mRNA for the p55, IL-2Rα. However, a brief exposure to IFN-γ was sufficient to induce IL-2Rα mRNA in ANA-1 macrophages. Flow cytometric analysis indicated that ANA-1 macrophages expressed low constitutive levels of IL-2Rα on their cell surface that were augmented after treatment of the cells with IFN-γ. Affinity binding and cross-linking of [125I]IL-2 to ANA-1 macrophages demonstrated that IL-2Rα and the p70-75, IL-2Rβ were both present on ANA-1 macrophages constitutively. IFN-γ increased the expression of IL-2Rα on ANA-1 macrophages but did not increase the expression of IL-2Rβ on these macrophages. Although IL-2 alone did not induce the tumoricidal activity of ANA-1 macrophages, IL-2 acted synergistically with IFN-γ to induce macrophage tumoricidal activity. These data demonstrate the expression of IL-2R on murine macrophage cell lines and establish the role of IL-2 as a costimulator of macrophage-mediated tumoricidal activity.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025184248&partnerID=8YFLogxK
M3 - Article
C2 - 2118153
AN - SCOPUS:0025184248
VL - 145
SP - 1719
EP - 1726
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -