Characterization of IL-2 receptor expression and function on murine macrophages

G. W. Cox, B. J. Mathieson, S. L. Giardina, L. Varesio

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the J2 (v-raf/v-myc) recombinant murine retrovirus. ANA-1 macrophages did not constitutively express detectable levels of mRNA for the p55, IL-2Rα. However, a brief exposure to IFN-γ was sufficient to induce IL-2Rα mRNA in ANA-1 macrophages. Flow cytometric analysis indicated that ANA-1 macrophages expressed low constitutive levels of IL-2Rα on their cell surface that were augmented after treatment of the cells with IFN-γ. Affinity binding and cross-linking of [125I]IL-2 to ANA-1 macrophages demonstrated that IL-2Rα and the p70-75, IL-2Rβ were both present on ANA-1 macrophages constitutively. IFN-γ increased the expression of IL-2Rα on ANA-1 macrophages but did not increase the expression of IL-2Rβ on these macrophages. Although IL-2 alone did not induce the tumoricidal activity of ANA-1 macrophages, IL-2 acted synergistically with IFN-γ to induce macrophage tumoricidal activity. These data demonstrate the expression of IL-2R on murine macrophage cell lines and establish the role of IL-2 as a costimulator of macrophage-mediated tumoricidal activity.

Original languageEnglish
Pages (from-to)1719-1726
Number of pages8
JournalJournal of Immunology
Volume145
Issue number6
Publication statusPublished - 1990

Fingerprint

Interleukin-2 Receptors
Macrophages
Interleukin-2
Cell Line
Messenger RNA
Retroviridae
Bone Marrow Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Cox, G. W., Mathieson, B. J., Giardina, S. L., & Varesio, L. (1990). Characterization of IL-2 receptor expression and function on murine macrophages. Journal of Immunology, 145(6), 1719-1726.

Characterization of IL-2 receptor expression and function on murine macrophages. / Cox, G. W.; Mathieson, B. J.; Giardina, S. L.; Varesio, L.

In: Journal of Immunology, Vol. 145, No. 6, 1990, p. 1719-1726.

Research output: Contribution to journalArticle

Cox, GW, Mathieson, BJ, Giardina, SL & Varesio, L 1990, 'Characterization of IL-2 receptor expression and function on murine macrophages', Journal of Immunology, vol. 145, no. 6, pp. 1719-1726.
Cox, G. W. ; Mathieson, B. J. ; Giardina, S. L. ; Varesio, L. / Characterization of IL-2 receptor expression and function on murine macrophages. In: Journal of Immunology. 1990 ; Vol. 145, No. 6. pp. 1719-1726.
@article{b05b8dc4035c43998ee92e14989a9839,
title = "Characterization of IL-2 receptor expression and function on murine macrophages",
abstract = "This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the J2 (v-raf/v-myc) recombinant murine retrovirus. ANA-1 macrophages did not constitutively express detectable levels of mRNA for the p55, IL-2Rα. However, a brief exposure to IFN-γ was sufficient to induce IL-2Rα mRNA in ANA-1 macrophages. Flow cytometric analysis indicated that ANA-1 macrophages expressed low constitutive levels of IL-2Rα on their cell surface that were augmented after treatment of the cells with IFN-γ. Affinity binding and cross-linking of [125I]IL-2 to ANA-1 macrophages demonstrated that IL-2Rα and the p70-75, IL-2Rβ were both present on ANA-1 macrophages constitutively. IFN-γ increased the expression of IL-2Rα on ANA-1 macrophages but did not increase the expression of IL-2Rβ on these macrophages. Although IL-2 alone did not induce the tumoricidal activity of ANA-1 macrophages, IL-2 acted synergistically with IFN-γ to induce macrophage tumoricidal activity. These data demonstrate the expression of IL-2R on murine macrophage cell lines and establish the role of IL-2 as a costimulator of macrophage-mediated tumoricidal activity.",
author = "Cox, {G. W.} and Mathieson, {B. J.} and Giardina, {S. L.} and L. Varesio",
year = "1990",
language = "English",
volume = "145",
pages = "1719--1726",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "6",

}

TY - JOUR

T1 - Characterization of IL-2 receptor expression and function on murine macrophages

AU - Cox, G. W.

AU - Mathieson, B. J.

AU - Giardina, S. L.

AU - Varesio, L.

PY - 1990

Y1 - 1990

N2 - This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the J2 (v-raf/v-myc) recombinant murine retrovirus. ANA-1 macrophages did not constitutively express detectable levels of mRNA for the p55, IL-2Rα. However, a brief exposure to IFN-γ was sufficient to induce IL-2Rα mRNA in ANA-1 macrophages. Flow cytometric analysis indicated that ANA-1 macrophages expressed low constitutive levels of IL-2Rα on their cell surface that were augmented after treatment of the cells with IFN-γ. Affinity binding and cross-linking of [125I]IL-2 to ANA-1 macrophages demonstrated that IL-2Rα and the p70-75, IL-2Rβ were both present on ANA-1 macrophages constitutively. IFN-γ increased the expression of IL-2Rα on ANA-1 macrophages but did not increase the expression of IL-2Rβ on these macrophages. Although IL-2 alone did not induce the tumoricidal activity of ANA-1 macrophages, IL-2 acted synergistically with IFN-γ to induce macrophage tumoricidal activity. These data demonstrate the expression of IL-2R on murine macrophage cell lines and establish the role of IL-2 as a costimulator of macrophage-mediated tumoricidal activity.

AB - This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the J2 (v-raf/v-myc) recombinant murine retrovirus. ANA-1 macrophages did not constitutively express detectable levels of mRNA for the p55, IL-2Rα. However, a brief exposure to IFN-γ was sufficient to induce IL-2Rα mRNA in ANA-1 macrophages. Flow cytometric analysis indicated that ANA-1 macrophages expressed low constitutive levels of IL-2Rα on their cell surface that were augmented after treatment of the cells with IFN-γ. Affinity binding and cross-linking of [125I]IL-2 to ANA-1 macrophages demonstrated that IL-2Rα and the p70-75, IL-2Rβ were both present on ANA-1 macrophages constitutively. IFN-γ increased the expression of IL-2Rα on ANA-1 macrophages but did not increase the expression of IL-2Rβ on these macrophages. Although IL-2 alone did not induce the tumoricidal activity of ANA-1 macrophages, IL-2 acted synergistically with IFN-γ to induce macrophage tumoricidal activity. These data demonstrate the expression of IL-2R on murine macrophage cell lines and establish the role of IL-2 as a costimulator of macrophage-mediated tumoricidal activity.

UR - http://www.scopus.com/inward/record.url?scp=0025184248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025184248&partnerID=8YFLogxK

M3 - Article

VL - 145

SP - 1719

EP - 1726

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 6

ER -