Characterization of mitochondrial DNA in primary cardiomyopathies

Antonella Bobba, Sergio Giannattasio, Angela Pucci, Rosa Lippolis, Clara Camaschella, Ersilia Marra

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

With the aim of studying the involvement of the mitochondrial genome in the impairment of heart function, mitochondrial DNA was analyzed by modified primer shift-polymerase chain reaction in a panel of young patients affected by primary cardiomyopathies. Mitochondrial DNA molecules harboring the 7436 bp deletion were specifically found in cardiomyopathic patients as compared with a panel of control subjects. The 4977 bp deletion was commonly detected among the subjects analyzed whereas none of the specific tRNA gene point mutations generally associated with the cardiomyopathic trait were detected. The presence of the 7436 bp deletion as a consequence of a premature aging of the heart muscle, secondary to heart dysfunction, is discussed.

Original languageEnglish
Pages (from-to)181-189
Number of pages9
JournalClinica Chimica Acta
Volume243
Issue number2
DOIs
Publication statusPublished - Dec 29 1995

Fingerprint

Cardiomyopathies
Mitochondrial DNA
Premature Aging
Mitochondrial Genome
Transfer RNA
Genes
Point Mutation
Myocardium
Polymerase chain reaction
Polymerase Chain Reaction
Muscle
Aging of materials
Molecules

Keywords

  • Dilated cardiomyopathy
  • Hypertrophic cardiomyopathy
  • Mitochondrial DNA
  • Primer shift-PCR

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Characterization of mitochondrial DNA in primary cardiomyopathies. / Bobba, Antonella; Giannattasio, Sergio; Pucci, Angela; Lippolis, Rosa; Camaschella, Clara; Marra, Ersilia.

In: Clinica Chimica Acta, Vol. 243, No. 2, 29.12.1995, p. 181-189.

Research output: Contribution to journalArticle

Bobba, A, Giannattasio, S, Pucci, A, Lippolis, R, Camaschella, C & Marra, E 1995, 'Characterization of mitochondrial DNA in primary cardiomyopathies', Clinica Chimica Acta, vol. 243, no. 2, pp. 181-189. https://doi.org/10.1016/0009-8981(95)06166-5
Bobba, Antonella ; Giannattasio, Sergio ; Pucci, Angela ; Lippolis, Rosa ; Camaschella, Clara ; Marra, Ersilia. / Characterization of mitochondrial DNA in primary cardiomyopathies. In: Clinica Chimica Acta. 1995 ; Vol. 243, No. 2. pp. 181-189.
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