Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA

Y. Levy, B. Gilburd, J. George, N. Del Papa, R. Mallone, M. Damianovich, M. Blank, A. Radice, Y. Renaudineau, P. Youinou, A. Wiik, F. Malavasi, P. L. Meroni, Y. Shoenfeld

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The IgG fraction of human anti-endothelial cell antibodies (AECA) obtained from a patient with Wegener's granulomatosis was used as immunogen to raise AECA mAb in mice selected among those which developed vasculitis-like lesions after immunization. Three mAb (BGM, 3C8 and 7G2), selected by cyto-ELISA and flow cytometry analyses, featured a specific reactivity with human umbilical vein endothelial cells (HUVEC) and the mouse endothelial cell line H5V; on the contrary, HEp2 cells, the murine melanoma B16 cell line, the extracellular matrix as well as several other antigens tested were not recognized. BGM mAb, an IgG3 precipitating a 70 kDa structure from HUVEC, was able to induce endothelial cells to secrete amounts of IL-6 significantly higher than irrelevant controls or mAb binding different endothelial antigens (i.e. CD31, CD29, ICAM-1 and HLA class I). BGM mAb induced significant levels of antibody-dependent cell cytotoxicity (13 ± 2.5 versus 0.6 ± 0.03%). To the best of our knowledge, BGM is the first murine mAb specific for human endothelial cells generated by idiotypic manipulation; secondly, its biological properties further support the notion of a pathogenic role for AECA in autoimmune-mediated diseases.

Original languageEnglish
Pages (from-to)861-868
Number of pages8
JournalInternational Immunology
Volume10
Issue number7
DOIs
Publication statusPublished - 1998

Fingerprint

Endothelial Cells
Human Umbilical Vein Endothelial Cells
Immunoglobulin G
CD31 Antigens
Antibody-Dependent Cell Cytotoxicity
Cell Line
Experimental Melanomas
Granulomatosis with Polyangiitis
Intercellular Adhesion Molecule-1
Vasculitis
Autoimmune Diseases
Extracellular Matrix
Immunization
Interleukin-6
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Antigens
anti-endothelial cell antibody

Keywords

  • Anti-endothelial antibodies
  • Autoimmunity
  • mAb

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA. / Levy, Y.; Gilburd, B.; George, J.; Del Papa, N.; Mallone, R.; Damianovich, M.; Blank, M.; Radice, A.; Renaudineau, Y.; Youinou, P.; Wiik, A.; Malavasi, F.; Meroni, P. L.; Shoenfeld, Y.

In: International Immunology, Vol. 10, No. 7, 1998, p. 861-868.

Research output: Contribution to journalArticle

Levy, Y, Gilburd, B, George, J, Del Papa, N, Mallone, R, Damianovich, M, Blank, M, Radice, A, Renaudineau, Y, Youinou, P, Wiik, A, Malavasi, F, Meroni, PL & Shoenfeld, Y 1998, 'Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA', International Immunology, vol. 10, no. 7, pp. 861-868. https://doi.org/10.1093/intimm/10.7.861
Levy, Y. ; Gilburd, B. ; George, J. ; Del Papa, N. ; Mallone, R. ; Damianovich, M. ; Blank, M. ; Radice, A. ; Renaudineau, Y. ; Youinou, P. ; Wiik, A. ; Malavasi, F. ; Meroni, P. L. ; Shoenfeld, Y. / Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA. In: International Immunology. 1998 ; Vol. 10, No. 7. pp. 861-868.
@article{2d358823869e45ccadcbdbd445414224,
title = "Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA",
abstract = "The IgG fraction of human anti-endothelial cell antibodies (AECA) obtained from a patient with Wegener's granulomatosis was used as immunogen to raise AECA mAb in mice selected among those which developed vasculitis-like lesions after immunization. Three mAb (BGM, 3C8 and 7G2), selected by cyto-ELISA and flow cytometry analyses, featured a specific reactivity with human umbilical vein endothelial cells (HUVEC) and the mouse endothelial cell line H5V; on the contrary, HEp2 cells, the murine melanoma B16 cell line, the extracellular matrix as well as several other antigens tested were not recognized. BGM mAb, an IgG3 precipitating a 70 kDa structure from HUVEC, was able to induce endothelial cells to secrete amounts of IL-6 significantly higher than irrelevant controls or mAb binding different endothelial antigens (i.e. CD31, CD29, ICAM-1 and HLA class I). BGM mAb induced significant levels of antibody-dependent cell cytotoxicity (13 ± 2.5 versus 0.6 ± 0.03{\%}). To the best of our knowledge, BGM is the first murine mAb specific for human endothelial cells generated by idiotypic manipulation; secondly, its biological properties further support the notion of a pathogenic role for AECA in autoimmune-mediated diseases.",
keywords = "Anti-endothelial antibodies, Autoimmunity, mAb",
author = "Y. Levy and B. Gilburd and J. George and {Del Papa}, N. and R. Mallone and M. Damianovich and M. Blank and A. Radice and Y. Renaudineau and P. Youinou and A. Wiik and F. Malavasi and Meroni, {P. L.} and Y. Shoenfeld",
year = "1998",
doi = "10.1093/intimm/10.7.861",
language = "English",
volume = "10",
pages = "861--868",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA

AU - Levy, Y.

AU - Gilburd, B.

AU - George, J.

AU - Del Papa, N.

AU - Mallone, R.

AU - Damianovich, M.

AU - Blank, M.

AU - Radice, A.

AU - Renaudineau, Y.

AU - Youinou, P.

AU - Wiik, A.

AU - Malavasi, F.

AU - Meroni, P. L.

AU - Shoenfeld, Y.

PY - 1998

Y1 - 1998

N2 - The IgG fraction of human anti-endothelial cell antibodies (AECA) obtained from a patient with Wegener's granulomatosis was used as immunogen to raise AECA mAb in mice selected among those which developed vasculitis-like lesions after immunization. Three mAb (BGM, 3C8 and 7G2), selected by cyto-ELISA and flow cytometry analyses, featured a specific reactivity with human umbilical vein endothelial cells (HUVEC) and the mouse endothelial cell line H5V; on the contrary, HEp2 cells, the murine melanoma B16 cell line, the extracellular matrix as well as several other antigens tested were not recognized. BGM mAb, an IgG3 precipitating a 70 kDa structure from HUVEC, was able to induce endothelial cells to secrete amounts of IL-6 significantly higher than irrelevant controls or mAb binding different endothelial antigens (i.e. CD31, CD29, ICAM-1 and HLA class I). BGM mAb induced significant levels of antibody-dependent cell cytotoxicity (13 ± 2.5 versus 0.6 ± 0.03%). To the best of our knowledge, BGM is the first murine mAb specific for human endothelial cells generated by idiotypic manipulation; secondly, its biological properties further support the notion of a pathogenic role for AECA in autoimmune-mediated diseases.

AB - The IgG fraction of human anti-endothelial cell antibodies (AECA) obtained from a patient with Wegener's granulomatosis was used as immunogen to raise AECA mAb in mice selected among those which developed vasculitis-like lesions after immunization. Three mAb (BGM, 3C8 and 7G2), selected by cyto-ELISA and flow cytometry analyses, featured a specific reactivity with human umbilical vein endothelial cells (HUVEC) and the mouse endothelial cell line H5V; on the contrary, HEp2 cells, the murine melanoma B16 cell line, the extracellular matrix as well as several other antigens tested were not recognized. BGM mAb, an IgG3 precipitating a 70 kDa structure from HUVEC, was able to induce endothelial cells to secrete amounts of IL-6 significantly higher than irrelevant controls or mAb binding different endothelial antigens (i.e. CD31, CD29, ICAM-1 and HLA class I). BGM mAb induced significant levels of antibody-dependent cell cytotoxicity (13 ± 2.5 versus 0.6 ± 0.03%). To the best of our knowledge, BGM is the first murine mAb specific for human endothelial cells generated by idiotypic manipulation; secondly, its biological properties further support the notion of a pathogenic role for AECA in autoimmune-mediated diseases.

KW - Anti-endothelial antibodies

KW - Autoimmunity

KW - mAb

UR - http://www.scopus.com/inward/record.url?scp=7344244338&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7344244338&partnerID=8YFLogxK

U2 - 10.1093/intimm/10.7.861

DO - 10.1093/intimm/10.7.861

M3 - Article

C2 - 9701024

AN - SCOPUS:7344244338

VL - 10

SP - 861

EP - 868

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 7

ER -