Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA

Y. Levy, B. Gilburd, J. George, N. Del Papa, R. Mallone, M. Damianovich, M. Blank, A. Radice, Y. Renaudineau, P. Youinou, A. Wiik, F. Malavasi, P. L. Meroni, Y. Shoenfeld

Research output: Contribution to journalArticlepeer-review

Abstract

The IgG fraction of human anti-endothelial cell antibodies (AECA) obtained from a patient with Wegener's granulomatosis was used as immunogen to raise AECA mAb in mice selected among those which developed vasculitis-like lesions after immunization. Three mAb (BGM, 3C8 and 7G2), selected by cyto-ELISA and flow cytometry analyses, featured a specific reactivity with human umbilical vein endothelial cells (HUVEC) and the mouse endothelial cell line H5V; on the contrary, HEp2 cells, the murine melanoma B16 cell line, the extracellular matrix as well as several other antigens tested were not recognized. BGM mAb, an IgG3 precipitating a 70 kDa structure from HUVEC, was able to induce endothelial cells to secrete amounts of IL-6 significantly higher than irrelevant controls or mAb binding different endothelial antigens (i.e. CD31, CD29, ICAM-1 and HLA class I). BGM mAb induced significant levels of antibody-dependent cell cytotoxicity (13 ± 2.5 versus 0.6 ± 0.03%). To the best of our knowledge, BGM is the first murine mAb specific for human endothelial cells generated by idiotypic manipulation; secondly, its biological properties further support the notion of a pathogenic role for AECA in autoimmune-mediated diseases.

Original languageEnglish
Pages (from-to)861-868
Number of pages8
JournalInternational Immunology
Volume10
Issue number7
DOIs
Publication statusPublished - 1998

Keywords

  • Anti-endothelial antibodies
  • Autoimmunity
  • mAb

ASJC Scopus subject areas

  • Immunology

Fingerprint

Dive into the research topics of 'Characterization of murine monoclonal anti-endothelial cell antibodies (AECA) produced by idiotypic manipulation with human AECA'. Together they form a unique fingerprint.

Cite this