Characterization of mycobacteria from a major Brazilian outbreak suggests that revision of the taxonomic status of members of the Mycobacterium chelonae-M. abscessus group is needed

Sylvia Cardoso Leao, Enrico Tortoli, Cristina Viana-Niero, Suely Yoko Mizuka Ueki, Karla Valeria Batista Lima, Maria Luiza Lopes, Jesus Yubero, Maria Carmen Menendez, Maria Jesus Garcia

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Abstract

An outbreak of postsurgical infections caused by rapidly growing mycobacteria has been ongoing in Brazil since 2004. The degrees of similarity of the rpoB and hsp65 sequences from the clinical isolates and the corresponding sequences from both the Mycobacterium massiliense and the M. bolletii type strains were above the accepted limit for interspecies variability, leading to conflicting identification results. Therefore, an extensive characterization of members of the M. chelonae-M. abscessus group was carried out. The M. abscessus, M. chelonae, M. immunogenum, M. massiliense, and M. bolletii type strains and a subset of clinical isolates were analyzed by biochemical tests, high-performance liquid chromatography, drug susceptibility testing, PCRrestriction enzyme analysis of hsp65 (PRA-hsp65), rpoB, and hsp65 gene sequencing and analysis of phylogenetic trees, DNA-DNA hybridization (DDH), and restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene (RFLP-16S rRNA). The clinical isolates and the M. abscessus, M. massiliense, and M. bolletii type strains could not be separated by phenotypic tests and were grouped in the phylogenetic trees obtained. The results of DDH also confirmed the >70% relatedness of the clinical isolates and the M. abscessus, M. massiliense, and M. bolletii type strains; and indistinguishable RFLP-16S rRNA patterns were obtained. On the contrary, the separation of clinical isolates and the M. abscessus, M. massiliense, and M. bolletii type strains from M. chelonae and M. immunogenum was supported by the results of PRA-hsp65, DDH, and RFLP-16S rRNA and by the rpoB and hsp65 phylogenetic trees. Taken together, these results led to the proposition that M. abscessus, M. massiliense, and M. bolletii represent a single species, that of M. abscessus. Two subspecies are also proposed, M. abscessus subsp. abscessus and M. abscessus subsp. massiliense, and these two subspecies can be distinguished by two different PRA-hsp65 patterns, which differ by a single HaeIII band, and by differences in their rpoB (3.4%) and hsp65 (1.3%) sequences.

Original languageEnglish
Pages (from-to)2691-2698
Number of pages8
JournalJournal of Clinical Microbiology
Volume47
Issue number9
DOIs
Publication statusPublished - Sep 2009

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Mycobacterium chelonae
Mycobacterium
Disease Outbreaks
DNA
Restriction Fragment Length Polymorphisms
Enzymes
rRNA Genes
Brazil
High Pressure Liquid Chromatography
Infection
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Microbiology (medical)

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Characterization of mycobacteria from a major Brazilian outbreak suggests that revision of the taxonomic status of members of the Mycobacterium chelonae-M. abscessus group is needed. / Leao, Sylvia Cardoso; Tortoli, Enrico; Viana-Niero, Cristina; Ueki, Suely Yoko Mizuka; Lima, Karla Valeria Batista; Lopes, Maria Luiza; Yubero, Jesus; Menendez, Maria Carmen; Garcia, Maria Jesus.

In: Journal of Clinical Microbiology, Vol. 47, No. 9, 09.2009, p. 2691-2698.

Research output: Contribution to journalArticle

Leao, Sylvia Cardoso ; Tortoli, Enrico ; Viana-Niero, Cristina ; Ueki, Suely Yoko Mizuka ; Lima, Karla Valeria Batista ; Lopes, Maria Luiza ; Yubero, Jesus ; Menendez, Maria Carmen ; Garcia, Maria Jesus. / Characterization of mycobacteria from a major Brazilian outbreak suggests that revision of the taxonomic status of members of the Mycobacterium chelonae-M. abscessus group is needed. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 9. pp. 2691-2698.
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